Figure 1: Isolation and identification of human fibroblasts and ASCs in vitro. Fibroblasts were spindle-shaped (a) and positive for immunofluorescence staining of vimentin (b) and negative for cytokeratin 15 (c). ASCs were identified by flow cytometry of mesenchymal stem cells markers (d1–d6) and multiple differentiations. Adipogenesis of ASCs was confirmed by Oil Red O staining (e) and gene expressions of PPARγ (g). Osteogenesis was confirmed by Alizarin red staining (f) and gene expression of Runx2 (h).