Reconstruction of the Fatty Acid Biosynthetic Pathway of Exiguobacterium antarcticum B7 Based on Genomic and Bibliomic Data

Table 2

Relationships between components of the E. antarcticum B7 fatty acid biosynthesis pathway following curation. The signals and indicate irreversible and reversible reactions, respectively.

Gene	Locus tag	EC number	Enzyme	Reaction	Fold change ()
accA	Eab7_2059	6.4.1.2	Acetyl-CoA carboxylase carboxyl transferase alpha subunit	ATP + acetyl-CoA + ADP + orthophosphate + malonyl-CoA	0.4562
accB	Eab7_0870	6.4.1.2	Acetyl-CoA carboxylase biotin-carboxyl carrier protein	ATP + acetyl-CoA + ADP + orthophosphate + malonyl-CoA	−0.05773
accC	Eab7_0871	6.4.1.2; 6.4.1.14	Acetyl-CoA carboxylase, biotin carboxylase subunit	ATP + acetyl-CoA + ADP + orthophosphate + malonyl-CoA	−0.5623
accD	Eab7_2060	6.4.1.2	Acetyl-CoA carboxylase carboxyl transferase beta subunit	ATP + acetyl-CoA + ADP + orthophosphate + malonyl-CoA	−0.2811
fabD	Eab7_1760	2.3.1.39	ACP S-malonyl transferase	Malonyl-CoA + ACP CoA + malonyl-(acp)	0.9448
fabH1	Eab7_1911	2.3.1.180	3-Oxoacyl-ACP synthase III	Acetyl-CoA + malonyl-(acp) acetoacetyl-(acp) + CoA + CO2	0.8512
fabF	Eab7_1910	2.3.1.179	3-Oxoacyl-ACP synthase II	Acetyl-(acp) + malonyl-(acp) acetoacetyl-(acp) + CO2 + ACP	0.6942
fabG	Eab7_1895	1.1.1.100	3-Oxoacyl-ACP reductase	Acetoacetyl-(acp) + NADPH + H^+   (R)-3-hydroxybutanoyl-(acp) + NADP^+	0.8523
fabZ	Eab7_2463	4.2.1.59	3-Hydroxyacyl-ACP dehydratase	(R)-3-hydroxybutanoyl-(acp) but-2-enoyl-(acp) + H2O	0.12902
fabI	Eab7_1885	1.3.1.9; 1.3.1.10	Enoyl-ACP reductase I	But-2-enoyl-(acp) + NADH + H^+   butyryl-(acp) + NAD^+	0.2969
—	Eab7_2235	1.14.19.2	Acyl-ACP desaturase	Hexadecanoyl-(acp) + acceptor_reduced + O2   hexadecenoyl-(acp) + acceptor + 2H2O	1.3768