Table 1: Dietary macro- and micronutrients and AGE levels.

Bio-ServHarlan Laboratories
AutoclavedNot autoclavedAutoclavedNot autoclaved

Mouse groupsGroups 1 & 5Groups 2 & 6Groups 3 & 7Groups 4 & 8
Diet designationHAGE-HFLAGE-HFHAGE-LFLAGE-LF
AGE (ng/g of chow)119.94922.969110.4934.105

Nutrients (%)
 Protein18.418.42424
 Fat7.27.244
 Carbohydrate58.658.646.1646.16
 Other (fiber)15.815.855
 Total calories3.93.93.823.82

Micronutrients (per kg of chow)
 Biotin0.2 mg/Kg0.2 mg/Kg0.38 mg/Kg0.38 mg/Kg
 Folate2.0 mg/Kg2.0 mg/Kg3.21 mg/Kg3.21 mg/Kg
 Niacin30.0 mg/Kg30.0 mg/Kg60.42 mg/Kg60.42 mg/Kg
 Pyridoxine6.0 mg/Kg6.0 mg/Kg14.13 mg/Kg14.13 mg/Kg
 Riboflavin6.0 mg/Kg6.0 mg/Kg7.89 mg/Kg7.89 mg/Kg
 Thiamin5.0 mg/Kg5.0 mg/Kg28.08 mg/Kg28.08 mg/Kg
 Vitamin A4000 IU4000 IU15.79 IH/g15.79 IH/g
 Vitamin B1225.0 mcg25.0 mcg51.2 mcg/Kg51.2 mcg/Kg
 Vitamin E75 IU75 IU94.72 IU/Kg94.72 IU/Kg
 Vitamin D31000 IU1000 IU2.98 IU/g2.98 IU/g

AGE: measured using CML- (-carboxymethyl-lysine-) sensitive ELISA.