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BioMed Research International
Volume 2016, Article ID 7893961, 8 pages
http://dx.doi.org/10.1155/2016/7893961
Research Article

Sperm DNA Fragmentation Index and Hyaluronan Binding Ability in Men from Infertile Couples and Men with Testicular Germ Cell Tumor

1Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland
2II Clinic of Urology, Medical University of Lodz, Lodz, Poland
3Infertility Clinic, Salve Medica, Lodz, Poland

Received 5 October 2016; Accepted 26 October 2016

Academic Editor: Janusz Blasiak

Copyright © 2016 Katarzyna Marchlewska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To investigate sperm DNA fragmentation and sperm functional maturity in men from infertile couples (IC) and men with testicular germ cell tumor (TGCT). Materials and Methods. Semen samples were collected from 312 IC men and 23 men with TGCT before unilateral orchiectomy and oncological treatment. The sperm chromatin dispersion test was performed to determine DNA fragmentation index (DFI) and the ability of sperm to bind with hyaluronan (HA) was assessed. Results. In comparison with the IC men, the men with TGCT had a higher percentage of sperm with fragmented DNA (median 28% versus 21%; ) and a lower percentage of HA-bound sperm (24% versus 66%; ). Normal results of both analyses were observed in 24% of IC men and 4% of men with TGCT. Negative Spearman’s correlations were found between DFI and the percentage of HA-bound sperm in the whole group and in IC subjects and those with TGCT analyzed separately. Conclusions. Approximately 76% of IC men and 96% with TGCT awaiting orchiectomy demonstrated DNA fragmentation and/or sperm immaturity. We therefore recommend sperm banking after unilateral orchiectomy, but before irradiation and chemotherapy; the use of such a deposit appears to be a better strategy to obtain functionally efficient sperms.