NRAMP1 Polymorphisms like Susceptibility Marker in Mexican Focus of Cutaneous Leishmaniasis

Table 2

Details of the polymorphisms genotyped and identified by PCR-RFLP in NRAMP1.

Polymorphisms	Localization	PCR product size	Restriction enzyme	Restriction enzyme products
−524G/C	Promoter region	585 bp	Hinf I	Allele 1 (G): 43 (2), 61, 62, 79, 297 bp; Allele 2 (C): 43, 61, 79, 105, 297 bp; G/C: 43, 61, 62, 79, 105, 297 bp
274C/T	Exon 3	216 bp	Mnl I	Allele 1 (C): 12, 37, 65, 102 bp; Allele 2 (T): 12, 37, 167 bp; C/T: 12, 37, 65, 102, 167 bp
823C/T	Exon 8	234 bp	Nar I	Allele 1 (C): 99, 135 bp; Allele 2 (T): 234 bp; C/T: 99, 135, 234 bp
D543N	Exon 15	240/244 bp	Ava II	Allele 1 (Asp): 39, 79, 122/126 bp; Allele 2 (Asn): 39, 201/205 bp; Asp/Asn: 39, 79, 122/126, 201/205 bp
1729 + 55del4	3′UTR	240/244 bp	Fok I	Allele 1 (+TGTG): 33, 211 bp; Allele 2 (−TGTG): 240; +/−: 33, 211, 240