Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression of Pseudomonas aeruginosa In Vitro

Table 1

Primer sequences for quantitative RT-PCR.

Gene	Primer	Amplicon (bp)
ToxA	Forward: GCCGATCTACACCATCGAGA; Reverse: CATCTCGTTGCTCTCGTGC	94
RhlA	Forward: TGATCACCAAGGACGACGAG; Reverse: GCCAGCAGCGTGGAGATAC	106
LasB	Forward: GACCCACAAGCTGTACATGAAG; Reverse: CCAGCGGATAGAACATGGTG	110
LasI	Forward: ACTCAGCCGTTTCGCCAT; Reverse: TCATCTTCTCCACGCCTACG	152
RhlI	Forward: ATTCTGGTCCAGCCTGCAA; Reverse: CTGGAGGATCACGCCGTT	109
RpoD	Forward: AGAGAAGGACGACGAGGAAGAAG; Reverse: GGCCAGGCCGGTGAGTTC	193