BioMed Research International / 2016 / Article / Tab 2

Research Article

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis

Table 2

Details of mutagenesis experiments showing combination of primers in the primer set for the intended base substitutions.

Primer setPrimersExpected size (bp)Base pair changesJoined full length DNA size (bp)Position of mutation on

1FGSP1 + RMP1387ATa aGg ATt cGc19251002–1005
RGSP1 + FMP11564
2FGSP1 + RMP2513CTa ATa CTg ATt19251125–1128
RGSP1 + FMP21444
3FGSP1 + RMP3931CCc ATa CCg ATt19251545–1548
RGSP1 + FMP31022
4FGSP1 + RMP41019aGa cGt19251639–1641
RGSP1 + FMP4923
5FGSP1 + RMP51166aGg aGa cGt cGc19251783–1788
RGSP1 + FMP5779
6FGSP1 + RMP61441CTa GAG aGa CGa CTg GAG cGt CGc19252052–2061
RGSP1 + FMP6512
7FGSP1 + RMP71649aGa ATG aGa aGa cGc ATG cGt cGc19252257–2268
RGSP1 + FMP7308
8FGSP2 + RMP8469TAt ATc tcc TTc TAc ATt agt TTt2685615–624
RGSP2 + FMP82248

Substituted bases are bold and written in lowercase.
of mutation on Stisa2 gene sequence (Accession: AY132997).