BioMed Research International / 2016 / Article / Tab 3

Research Article

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis

Table 3

Results of primary and secondary PCR reactions with different primer sets.

Primer setFirst primary PCRSecond primary PCRSecondary PCR
Estimated band sizeOptimization required?Gel excision required?Estimated band sizeOptimization required?Gel excision required?Estimated band size

1400NoNo1500NoYes1900
2500YesYes1400NoYes1900
3900NoNo1000NoNo1900
41000NoNo900NoNo1900
51100NoNo800NoYes1900
61400YesYes500NoNo1900
71600NoNo300NoNo1900
8450NoNo2250NoNo2700