Research Article

Expression of Progesterone Receptor Membrane Component 1 (PGRMC1), Progestin and AdipoQ Receptor 7 (PAQPR7), and Plasminogen Activator Inhibitor 1 RNA-Binding Protein (PAIRBP1) in Glioma Spheroids In Vitro

Figure 6

Demonstration of the colocalization of PGRMC1 (green) and PAIRBP1 (red) proteins in U-87 MG and LN-229 glioma cell spheroids by means of double-immunofluorescence. Regions of colocalization are demonstrated by yellow color indicating merged red and green signals. In both cell lines, nuclear PGRMC1 staining (white arrows) was observed. Nuclear counterstaining was performed with 4′,6-diamidino-2-phenylindole (blue). Scale bar 20 μm.