The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b⁺Gr1⁺ Cells in a Transgenic Mouse Model

Figure 3

Impaired migration of dendritic cells from the skin to the lymph nodes in K14E7 mice after sensitization with FITC. Shaved ventral mouse skin from K14E7 and NT mice was painted with fluorescein isothiocyanate (FITC) or vehicle control alone (NO FITC). After 24 hours, the inguinal, popliteal, axillary, and brachial lymph nodes were obtained and disaggregated. FITC⁺/CD11c⁺ cells were analyzed using flow cytometry. Experiments were performed in duplicate at least 2 times ( mice per experimental group). Significant differences are indicated with asterisks: .