Table 1: Number of isolates and strains of the identified Arcobacter species found from wastewater using the two molecular methods (m-PCRand 16S rRNA-RFLP) in parallel.

SpeciesNumber of isolates (%)Number of strains (%)% genetic diversityMolecular identification
/16S

A. butzleri (Ab)354 (54.4)247 (58.3)69.8%Ab/Ab
A. cryaerophilus (Ac)226 (34.7)150 (35.4)66.4%Ac/Ac
A. thereius (At)37 (5.7)9 (2.1)24.3%Ac/Ab
A. skirrowii (As)16 (2.5)5 (1.2)31.3%As/As
A. (Ad)12 (1.8)8 (1.9)66.7%~230 bp/Ad
A. ellisii (Ae)3 (0.5)2 (0.5)66.7%Ac/Ae
A. (Aclo)2 (0.3)2 (0.5)100%Ac/Aclo
A. nitrofigilis (An)1 (0.2)1 (0.2)100%As/An

Total65142465.1%

The isolates were genotyped with ERIC-PCR to determine the ones that showed the same ERIC-pattern and therefore belonged to the same strain. et al. [19] and et al. [20]. Results of the RFLP were verified by sequencing the rpoB gene. species recognized on the basis of the new RFLP pattern described by Figueras et al [20]. species recognized on the basis of the new RFLP pattern and described by Levican et al. [21].