¹²⁵I Seeds Radiation Induces Paraptosis-Like Cell Death via PI3K/AKT Signaling Pathway in HCT116 Cells

Figure 7

¹²⁵I seeds radiation activated PI3K/AKT signaling pathway. Cells were harvested 48 h after exposure to the gradually increased dose of ¹²⁵I seeds radiation. The expressions of AKT, p-Akt (Thr308), and p-Akt (Ser473) were determined by western blotting analysis. (a) Equal amounts of cell lysates from control and irradiated cells were analyzed. The expressions of AKT and p-Akt (Thr308 and Ser473) were analyzed by western analysis with corresponding antibody. -Actin was evaluated as a loading control. (b)-(c) The intensity levels of the protein bands of p-Akt (Thr308) (b) and p-Akt (Ser473) (c) were quantified using Photoshop software. The data are normalized to AKT and presented as means ± SD, .

(a)

(b)

(c)