¹²⁵I Seeds Radiation Induces Paraptosis-Like Cell Death via PI3K/AKT Signaling Pathway in HCT116 Cells

Figure 8

Ly294002 inhibited the phosphorylation of AKT induced by ¹²⁵I seeds radiation. HCT116 cells were pretreated with or without Ly294002 before exposure to 2 Gy of ¹²⁵I seeds radiation. Cells lysates were harvested 48 h after irradiation. (a) The expression of AKT and p-Akt (Thr308 and Ser473) was analyzed by western blotting. (b)-(c) The intensity levels of the protein bands of p-Akt (Thr308) (b) and p-Akt (Ser473) (c) were quantified using Photoshop analysis software. The data are normalized to AKT and presented as means ± SD, .

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