The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca2+]i
Photomicrographs illustrating loading of astrocytes with fura-2 and imaged at 380 nm excitation and 510 nm emission. (a) Astrocytes in the retinal whole mount preparation (in situ) loaded with fura-2 by electroporation. Numerous astrocytes are loaded; some examples are highlighted within boxes. A blood vessel can been seen with fura-2-loaded presumptive perivascular endfeet (arrows). (b) Astrocytes in vitro loaded with fura-2. Scale bar (for both (a) and (b)) 50 μm.