Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo

Figure 2

Changes in senescent cells induced by extracellular matrix (ECM) coating. (a) Morphological changes and population doubling time in senescent cells cultivated on a poly-L-lysine- (PLL-) coated plate were observed compared to cells cultured on an uncoated plate (magnification: 100x, scale bar = 200 μm). (b) Cell cycle analysis. Cells were removed from the culture well, stained for DNA with propidium iodide (PI), and analysed by flow cytometry. (c) Senescence associated -gal assay of MSCs cultured on uncoated and PLL-coated plates. One representative of three independent experiments is shown. The number of -gal positive cells was enumerated. The data represent the mean ± standard deviation of three experiments (magnification: 200x, scale bar = 100 μm). .

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