Research Article

Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots

Table 8

Comparison of clusters with previously identified L. sericata S1 peptidases.

GenBank IDNameProposed functionReferenceClusterPeptidase speciesMEROPS ID

CAS92770Chymotrypsin IWound debridement[15] LST_LS005873Chymotrypsin m-type 2 (insect) (L. cuprina)S01.168

KT158461Jonah-like chymotrypsinPlasma clotting/debridement[17]LST_LS007060Jonah 65Aiv (D. melanogaster)S01.B05

AAA17384 Sericase/trypsin Fibrinolysis/debridement [44]LST_LS007476CG7542 protein (D. melanogaster)S01.B07
[185]CG7542 protein (D. melanogaster)S01.B07

AEX33291  Putative salivary trypsin  Not described  [198]  LST_LS016882CLIP-domain prophenoloxidase activating factor (C. rubecula)S01.960
LST_LS005035CLIP-domain prophenoloxidase activating factor (C. rubecula)S01.960
LST_LS005470CLIP-domain prophenoloxidase activating factor (C. rubecula)S01.960
LST_LS016440CLIP-domain prophenoloxidase activating factor (C. rubecula)S01.960
LST_LS005552CLIP-domain prophenoloxidase activating factor (C. rubecula)S01.960
LST_LS005028CLIP-domain prophenoloxidase activating factor (C. rubecula)S01.960

AEX33294Putative salivary trypsinNot described[198]LST_LS003737IP10861 protein (D. melanogaster)S01.B61

AEX33290 Putative chymotrypsin Not described [198] LST_LS016062CG10477 protein (D. melanogaster)S01.B12
LST_LS007621CG10477 protein (D. melanogaster)S01.B12

AJN88395DebrilaseDebridementPatent # US 8623810LST_LS015273CG17571 protein (D. melanogaster)S01.A85

FG360474 Upregulated upon immune challenge [18] LST_LS007407CG11911 protein (D. melanogaster)S01.B68
LST_LS015021CG11911 protein (D. melanogaster)S01.B68

FG360505Upregulated upon immune challenge[18]LST_LS015269Jonah 65Aiv (D. melanogaster)S01.B05

FG360512Upregulated upon immune challenge[18]LST_LS008125Subfamily S1A non-peptidase homologuesS01.UNA

FG360521Upregulated upon immune challenge[18]LST_LS008080CG9676 protein (D. melanogaster)S01.A89

FG360523Upregulated upon immune challenge[18]LST_LS003034Trypsin alpha (insect) (D. melanogaster)S01.110

FG360527Upregulated upon immune challenge[18]LST_LS007843CG17571 protein (D. melanogaster)S01.A85

FG360528 Upregulated upon immune challenge[18] LST_LS015518CG16749 protein (D. melanogaster)S01.B48
LST_LS007441CG16749 protein (D. melanogaster)S01.B48

FG360529Upregulated upon immune challenge[18]LST_LS007476CG7542 protein (D. melanogaster)S01.B07