Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots

Table 9

Genes and qRT-PCR primers evaluated in this study.

Clan	Family	MEROPS ID	Peptidase sp.	Cluster	Primer sequences (5′- 3′)	(bp)	(%)
AA	A1	A01.092	CAD2 peptidase; (M. domestica)	LST_LS005916	5′-GCTGCCAAGCTATTGCTGAT-3′	75	102	0.997
					5′-CGGCTTGAATGTTTTCGTATT-3′
CA	C1	C01.A27	CG12163 protein; (D. melanogaster)	LST_LS006048	5′- TTTCACCGGTAATCGCAAAT -3′	66	105	0.997
					5′- GCAGCCTTTTGACGATGTTT -3′
MA	M1	M01.024	ERAP2 aminopeptidase	LST_LS004632	5′- CCATTCCGTCCGAT TCTT -3′	68	98	0.999
					5′- ATCGGCATACTGGGCAAA -3′
PB	T1	T01.976	Proteasome subunit alpha 1	LST_LS006843	5′- TCTTCACGCTCTCAAGACGA -3′	61	103	0.993
					5′- GGTCTTGTGTTTCGCCATCT -3′
PA	S1	S01.993	Testis-specific protein; 50 (H. sapiens)	LST_LS010066	5′- TATGGGCAGCCCTAACGA -3′	60	104	0.998
					5′- AAAGAACGGAGAGCATCACCT -3′

Length of amplicon.
Quantitative qRT-PCR efficiency.
Coefficient of determination.