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BioMed Research International
Volume 2016 (2016), Article ID 8329513, 9 pages
http://dx.doi.org/10.1155/2016/8329513
Research Article

Functions of thga1 Gene in Trichoderma harzianum Based on Transcriptome Analysis

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received 16 February 2016; Revised 22 May 2016; Accepted 19 July 2016

Academic Editor: Hongwei Wang

Copyright © 2016 Qing Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. Vinale, E. L. Ghisalberti, K. Sivasithamparam et al., “Factors affecting the production of Trichoderma harzianum secondary metabolites during the interaction with different plant pathogens,” Letters in Applied Microbiology, vol. 48, no. 6, pp. 705–711, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. P. K. Mukherjee, B. A. Horwitz, A. Herrera-Estrella, M. Schmoll, and C. M. Kenerley, “Trichoderma research in the genome era,” Annual Review of Phytopathology, vol. 51, pp. 105–129, 2013. View at Publisher · View at Google Scholar · View at Scopus
  3. Y. Hu, G. Liu, Z. Li, Y. Qin, Y. Qu, and X. Song, “G protein-cAMP signaling pathway mediated by PGA3 plays different roles in regulating the expressions of amylases and cellulases in Penicillium decumbens,” Fungal Genetics and Biology, vol. 58-59, pp. 62–70, 2013. View at Publisher · View at Google Scholar · View at Scopus
  4. P. K. Mukherjee, J. Latha, R. Hadar, and B. A. Horwitz, “Role of two G-protein alpha subunits, TgaA and TgaB, in the antagonism of plant pathogens by Trichoderma virens,” Applied and Environmental Microbiology, vol. 70, no. 1, pp. 542–549, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. M. Omann and S. Zeilinger, “How a mycoparasite employs G-protein signaling: using the example of Trichoderma,” Journal of Signal Transduction, vol. 2010, Article ID 123126, 8 pages, 2010. View at Publisher · View at Google Scholar
  6. L. Li, S. J. Wright, S. Krystofova, G. Park, and K. A. Borkovich, “Heterotrimeric G protein signaling in filamentous fungi,” Annual Review of Microbiology, vol. 61, pp. 423–452, 2007. View at Publisher · View at Google Scholar · View at Scopus
  7. B. A. Horwitz, A. Sharon, S.-W. Lu et al., “A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation,” Fungal Genetics and Biology, vol. 26, no. 1, pp. 19–32, 1999. View at Publisher · View at Google Scholar · View at Scopus
  8. B. Reithner, K. Brunner, R. Schuhmacher et al., “The G protein α subunit Tga1 of Trichoderma atroviride is involved in chitinase formation and differential production of antifungal metabolites,” Fungal Genetics and Biology, vol. 42, no. 9, pp. 749–760, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. Z. Liu, Cloning and functional characterization of Thga1 gene of Trichoderma harzianum Th-33 [Thesis], Chinese Academy of Agricultural Sciences, 2013.
  10. Q. Sun, X. Jiang, L. Pang, L. Wang, and M. Li, “The genome sequence of Trichoderma harzianum Th-33,” Chinese Journal of Biological Control, vol. 32, no. 2, pp. 205–214, 2016. View at Google Scholar
  11. D. Kim, G. Pertea, C. Trapnell, H. Pimentel, R. Kelley, and S. L. Salzberg, “TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions,” Genome Biology, vol. 14, no. 4, article R36, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. C. Trapnell, B. A. Williams, G. Pertea et al., “Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation,” Nature Biotechnology, vol. 28, no. 5, pp. 511–515, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. Y. Benjamini and D. Yekutieli, “The control of the false discovery rate in multiple testing under dependency,” The Annals of Statistics, vol. 29, no. 4, pp. 1165–1188, 2001. View at Publisher · View at Google Scholar · View at Zentralblatt MATH · View at MathSciNet · View at Scopus
  14. P. Y. Muller, H. Janovjak, A. R. Miserez, and Z. Dobbie, “Processing of gene expression data generated by quantitative real-time RT-PCR,” BioTechniques, vol. 32, no. 6, pp. 1372–1379, 2002. View at Google Scholar · View at Scopus
  15. A. Conesa and S. Götz, “Blast2GO: a comprehensive suite for functional analysis in plant genomics,” International Journal of Plant Genomics, vol. 2008, Article ID 619832, 12 pages, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. H. E. Hamm, “The many faces of G protein signaling,” The Journal of Biological Chemistry, vol. 273, no. 2, pp. 669–672, 1998. View at Publisher · View at Google Scholar · View at Scopus
  17. E. J. Neer, “Heterotrimeric C proteins: organizers of transmembrane signals,” Cell, vol. 80, no. 2, pp. 249–257, 1995. View at Publisher · View at Google Scholar · View at Scopus
  18. S. Gruber, M. Omann, and S. Zeilinger, “Comparative analysis of the repertoire of G protein-coupled receptors of three species of the fungal genus Trichoderma,” BMC Microbiology, vol. 13, no. 1, article 108, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. K. A. Borkovich, L. A. Alex, O. Yarden et al., “Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism,” Microbiology and Molecular Biology Reviews, vol. 68, no. 1, pp. 1–108, 2004. View at Publisher · View at Google Scholar · View at Scopus
  20. R. D. Kulkarni, M. R. Thon, H. Pan, and R. A. Dean, “Novel G-protein-coupled receptor-like proteins in the plant pathogenic fungus Magnaporthe grisea,” Genome Biology, vol. 6, no. 3, p. R24, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. D. Tisch and M. Schmoll, “Targets of light signalling in Trichoderma reesei,” BMC Genomics, vol. 14, no. 1, article 657, 2013. View at Publisher · View at Google Scholar · View at Scopus
  22. T. M. DeZwaan, A. M. Carroll, B. Valent, and J. A. Sweigard, “Magnaporthe grisea Pth11p is a novel plasma membrane protein that mediates appressorium differentiation in response to inductive substrate cues,” Plant Cell, vol. 11, no. 10, pp. 2013–2030, 1999. View at Publisher · View at Google Scholar · View at Scopus
  23. S. Muthumeenakshi, S. Sreenivasaprasad, C. W. Rogers, M. P. Challen, and J. M. Whipps, “Analysis of cDNA transcripts from Coniothyrium minitans reveals a diverse array of genes involved in key processes during sclerotial mycoparasitism,” Fungal Genetics and Biology, vol. 44, no. 12, pp. 1262–1284, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. S. M. Byrne and C. S. Huffman, “Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomyces pombe,” Journal of Cell Science, vol. 105, no. 4, pp. 1095–1100, 1993. View at Google Scholar · View at Scopus
  25. M. Mukherjee, P. K. Mukherjee, and S. P. Kale, “cAMP signalling is involved in growth, germination, mycoparasitism and secondary metabolism in Trichoderma virens,” Microbiology, vol. 153, no. 6, pp. 1734–1742, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. J. M. Steyaert, R. J. Weld, A. Mendoza-Mendoza, and A. Stewart, “Reproduction without sex: conidiation in the filamentous fungus Trichoderma,” Microbiology, vol. 156, no. 10, pp. 2887–2900, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Lafon, J.-A. Seo, K.-H. Han, J.-H. Yu, and C. D'Enfert, “The heterotrimeric G-protein GanB(α)-SfaD(β)-GpgA(γ) is a carbon source sensor involved in early cAMP-dependent germination in Aspergillus nidulans,” Genetics, vol. 171, no. 1, pp. 71–80, 2005. View at Publisher · View at Google Scholar · View at Scopus
  28. Z. Zhao, H. Liu, C. Wang, and J. R. Xu, “Comparative analysis of fungal genomes reveals different plant cell wall degrading capacity in fungi,” BMC Genomics, vol. 14, no. 6, article 274, 2014. View at Google Scholar
  29. C. P. Kubicek, A. Herrera-Estrella, V. Seidl-Seiboth et al., “Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma,” Genome Biology, vol. 12, no. 4, article R40, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. L. Atanasova, S. L. Crom, S. Gruber et al., “Comparative transcriptomics reveals different strategies of Trichoderma mycoparasitism,” BMC Genomics, vol. 14, no. 1, article 121, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. M. M. Babu, N. M. Luscombe, L. Aravind, M. Gerstein, and S. A. Teichmann, “Structure and evolution of transcriptional regulatory networks,” Current Opinion in Structural Biology, vol. 14, no. 3, pp. 283–291, 2004. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Nitta, T. Furukawa, Y. Shida et al., “A new Zn(II)2Cys6-type transcription factor BglR regulates β-glucosidase expression in Trichoderma reesei,” Fungal Genetics and Biology, vol. 49, no. 5, pp. 388–397, 2012. View at Publisher · View at Google Scholar · View at Scopus
  33. T. J. Schoberle, C. K. Nguyen-Coleman, J. Herold et al., “A novel C2H2 transcription factor that regulates gliA expression interdependently with GliZ in Aspergillus fumigatus,” PLoS Genetics, vol. 10, no. 5, Article ID e1004336, 2014. View at Publisher · View at Google Scholar · View at Scopus
  34. A. J. Clutterbuck, “A mutational analysis of conidial development in Aspergillus nidulans,” Genetics, vol. 63, no. 2, pp. 317–327, 1969. View at Google Scholar · View at Scopus
  35. H. Son, Y.-S. Seo, K. Min et al., “A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum,” PLoS Pathogens, vol. 7, no. 10, Article ID e1002310, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. C. S. Gronover, D. Kasulke, P. Tudzynski, and B. Tudzynski, “The role of G protein alpha subunits in the infection process of the gray mold fungus Botrytis cinerea,” Molecular Plant-Microbe Interactions, vol. 14, no. 11, pp. 1293–1302, 2001. View at Publisher · View at Google Scholar · View at Scopus
  37. D. Ribitsch, E. H. Acero, A. Przylucka et al., “Enhanced cutinase-catalyzed hydrolysis of polyethylene terephthalate by covalent fusion to hydrophobins,” Applied and Environmental Microbiology, vol. 81, no. 11, pp. 3586–3592, 2015. View at Publisher · View at Google Scholar · View at Scopus
  38. G. C. Segers, J. C. Regier, and D. L. Nuss, “Evidence for a role of the regulator of G-protein signaling protein CPRGS-1 in Gα subunit CPG-1-mediated regulation of fungal virulence, conidiation, and hydrophobin synthesis in the chestnut blight fungus Cryphonectria parasitica,” Eukaryotic Cell, vol. 3, no. 6, pp. 1454–1463, 2004. View at Publisher · View at Google Scholar · View at Scopus
  39. B. Črešnar and Š. Petrič, “Cytochrome P450 enzymes in the fungal kingdom,” Biochimica et Biophysica Acta, vol. 1814, no. 1, pp. 29–35, 2011. View at Publisher · View at Google Scholar · View at Scopus
  40. P. K. Mukherjee, B. A. Horwitz, and C. M. Kenerley, “Secondary metabolism in Trichoderma—a genomic perspective,” Microbiology, vol. 158, no. 1, pp. 35–45, 2012. View at Publisher · View at Google Scholar · View at Scopus
  41. D. Hoffmeister and N. P. Keller, “Natural products of filamentous fungi: enzymes, genes, and their regulation,” Natural Product Reports, vol. 24, no. 2, pp. 393–416, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. N. P. Keller, G. Turner, and J. W. Bennett, “Fungal secondary metabolism—from biochemistry to genomics,” Nature Reviews Microbiology, vol. 3, no. 12, pp. 937–947, 2005. View at Publisher · View at Google Scholar · View at Scopus
  43. A. Osbourn, “Secondary metabolic gene clusters: evolutionary toolkits for chemical innovation,” Trends in Genetics, vol. 26, no. 10, pp. 449–457, 2010. View at Publisher · View at Google Scholar · View at Scopus