Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing

Figure 1

Experimental outline of RNA-seq studies of production CHO cell lines. The tested CHO cell lines expressing mAb were propagated in suspension. Cell pellets were isolated and RNA samples were subsequently extracted. Reverse transcription was performed on the RNA samples and certain genes of interest were amplified from cDNAs. After library preparation, the product was sequenced on Illumina HiSeq2000. Details of data analysis are described in Section 3.