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BioMed Research International
Volume 2016, Article ID 8356435, 8 pages
Research Article

Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing

1Biologics & Vaccines, Merck Research Laboratories, Kenilworth, NJ 07033, USA
2Biology & Genetics Informatics, Merck Research Labs IT, Merck & Co, Boston, MA 02115, USA
3Discovery Pharmacogenomics, Merck Research Laboratories, Kenilworth, NJ 07033, USA

Received 18 November 2015; Revised 4 February 2016; Accepted 21 February 2016

Academic Editor: Jorge F. B. Pereira

Copyright © 2016 Siyan Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Details on sequences of primers and amplified regions are provided in Supplementary Materials. In addition, mutations with signal above 0.1% in all four genes are summarized in a table. Figure S2 provides coverage of light chain sequence for all samples in the main study. Figure S3 to S5 provides error:error plots comparing the replicates of the reference sample from the feasibility study, the re-sults from two replicates of two different spiking levels (0.1% and 0.5%) from the feasibility study and the replicates of the baseline sample from the main study.

  1. Supplementary Material