Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays

Figure 2

Analytical specificity of the PCR assay for detection of (a) STY0201 and (b) STY0307 genes (representative figures). Lane: M = 100 bp DNA ladder (Promega); N = negative control; 1 = S. Typhi; 2 = S. Paratyphi A; 3 = S. Paratyphi B; 4 = S. Paratyphi C; 5 = S. Enteritidis; 6 = S. Typhimurium; 7 = S. Weltevreden; 8 = S. Agona; 9 = S. Heidelberg; 10 = S. Poona; 11 = S. Hadar; 12 = S. Braenderup; 13 = S. Albany; 14 = S. Oslo; 15 = S. Kibi; 16 = S. Newport; 17 = S. Tshiongwe; 18 = S. Uppsala; 19 = S. Richmond; 20 = S. Bardo; 21 = S. Emek; 22 = S. Kissi; 23 = S. Virchow; 24 = S. Bordeaux; 25 = S. Regent; 26 = S. Java; 27 = S. Farsta; 28 = Shigella dysenteriae; 29 = Shigella flexneri; 30 = Shigella sonnei; 31 = Shigella boydii; 32 = Vibrio cholerae; 33 = Enterohemorrhagic Escherichia coli; 34 = Enteropathogenic Escherichia coli; 35 = Aeromonas hydrophila; 36 = Yersinia enterocolitica; and 37 = Klebsiella pneumonia. The PCR amplicon sizes for genes 16S rRNA, STY0201, and STY0307 were 1326 bp, 1176 bp, and 732 bp, respectively.

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