Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays

Table 1

List of primers targeting S. Typhi-specific genes for the development of 6 PCR assays.

Target genes	Primer labels	Primer sequences (5′-3′)	Target lengths (bp)
STY0201	0201F	ATGCTTTTAAAAAACACAACATGG	1176
	0201R	TTACGGATAGGTGATTGAAAATTG
STY0307	0307F	ATGAAACCTTTATTCTCAGTGC	495
	0307R	TTAGCGTAATTCCCAGAACC
STY0322	0322F	ATGAAATATAAAAAAATAAGAG	678
	0322R	CTATGGATTCATTTCCATTTC
STY0326	0326F	ATGAATACGAATAATTCACC	261
	0326R	TTACCCTCCCCATGTCAC
STY2020	2020F	ATGCCTGTTATGCATAATTG	429
	2020R	TTATGCTGTTAACGAGTCGTC
STY2021	2021F	ATGAGTTTAGCGCAGCCTAAATCC	732
	2021R	TTAGAAGTCTCCTGCCTGGAAAC
16S	16SF	CAGGCCTAACACATGCAAGTC	1362
	16SR	GGGCGGTGTGTACAAGGC

ribosomal RNA gene served as internal amplification control (IAC) [24].
F represents forward primer.
R represents reverse primer.