BioMed Research International

Research Article

Identification of Five Novel Salmonella Typhi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays

Table 2

Evaluation of the specificities of the 6 target genes for identification of S. Typhi using PCR (total of 111 clinical isolates).
Test bacteria strainsPositive PCR amplification for each target gene
STY0201STY0307STY0322STY0326STY2020STY2021
S. Typhi ()39/3939/3939/3939/3939/3939/39
S. Paratyphi A ()0/100/100/100/100/100/10
S. Paratyphi B ()0/100/100/100/100/100/10
S. Paratyphi C ()0/10/10/10/10/10/1
S. Enteritidis ()0/100/100/100/100/100/10
S. Typhimurium ()0/100/100/100/100/100/10
S. Weltevreden ()0/10/10/10/10/10/1
S. Agona ()0/10/10/10/10/10/1
S. Hadar ()0/10/10/10/10/10/1
S. Heidelberg ()0/10/10/10/10/10/1
S. Poona ()0/10/10/10/10/10/1
S. Braenderup ()0/10/10/10/10/10/1
S. Albany ()0/10/10/10/10/10/1
S. Oslo ()1/10/10/10/10/10/1
S. Kibi ()0/10/10/10/10/10/1
S. Newport ()0/10/10/10/10/10/1
S. Tshiongwe ()0/10/10/10/10/10/1
S. Uppsala ()0/10/10/10/10/10/1
S. Richmond ()0/10/10/10/10/10/1
S. Bardo ()0/10/10/10/10/10/1
S. Emek ()0/10/10/10/10/10/1
S. Kissi ()1/10/10/10/10/10/1
S. Virchow ()0/10/10/10/10/10/1
S. Bordeaux ()0/10/10/10/10/10/1
S. Regent ()0/10/10/10/10/10/1
S. Java ()0/10/10/10/10/10/1
S. Farsta ()0/10/10/10/10/10/1
Shigella dysenteriae ()0/10/10/10/10/10/1
Shigella flexneri ()0/10/10/10/10/10/1
Shigella sonnei ()0/10/10/10/10/10/1
Shigella boydii ()0/10/10/10/10/10/1
Vibrio cholerae ()0/10/10/10/10/10/1
Enterohemorrhagic E. coli ()0/10/10/10/10/10/1
Enteropathogenic E. coli ()0/10/10/10/10/10/1
Aeromonas hydrophila ()0/10/10/10/10/10/1
Yersinia enterocolitica ()0/10/10/10/10/10/1
Klebsiella pneumoniae ()0/10/10/10/10/10/1