Activated Müller Cells Involved in ATP-Induced Upregulation of P2X₇ Receptor Expression and Retinal Ganglion Cell Death

Figure 3

ATP-induced changes in P2X₇R protein expression in cultured rat RGCs. (a) Immunofluorescence labeling showing the changes in P2X₇R protein expression in control (Ctr) (a1) and ATP-treated cells for different times (2–48 h) ((a2)–(a5)). (b) Immunofluorescence labeling showing the expression of Brn3a, a RGC marker. (c) The corresponding merged images. Scale bar, 20 μm for all images. (d) Representative immunoblots showing the changes of P2X₇R protein expression in control (Ctr) and ATP-treated cells for different times (2–48 h). (e) Bar charts summarizing the average densitometric quantification of immunoreactive bands of P2X₇R protein levels under different conditions. for each group. versus Ctr.

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