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BioMed Research International
Volume 2016 (2016), Article ID 9140541, 12 pages
http://dx.doi.org/10.1155/2016/9140541
Research Article

Therapeutic Effects of CUR-Activated Human Umbilical Cord Mesenchymal Stem Cells on 1-Methyl-4-phenylpyridine-Induced Parkinson’s Disease Cell Model

1Neurology Department of General Hospital of Jinan Military Region, Jinan, Shandong 250031, China
2The Neurology Department, The 148th Hospital, Zibo, Shandong 255300, China
3Sanbo Brain Hospital Capital Medical University, Haidian District, Beijing 100093, China
4Medical School of Henan University, Zhengzhou, Henan 475000, China
5Neurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, China
6Department of Medicine, The University of Chicago, Chicago, IL 60637, USA

Received 23 November 2015; Revised 2 March 2016; Accepted 27 March 2016

Academic Editor: Nicola Simola

Copyright © 2016 Li Jinfeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study is to evaluate the therapeutic effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSC) activated by curcumin (CUR) on PC12 cells induced by 1-methyl-4-phenylpyridinium ion (MPP+), a cell model of Parkinson’s disease (PD). The supernatant of hUC-MSC and hUC-MSC activated by 5 µmol/L CUR (hUC-MSC-CUR) were collected in accordance with the same concentration. The cell proliferation and differentiation potential to dopaminergic neuronal cells and antioxidation were observed in PC12 cells after being treated with the above two supernatants and 5 µmol/L CUR. The results showed that the hUC-MSC-CUR could more obviously promote the proliferation and the expression of tyrosine hydroxylase (TH) and microtubule associated protein-2 (MAP2) and significantly decreased the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in PC12 cells. Furtherly, cytokines detection gave a clue that the expression of IL-6, IL-10, and NGF was significantly higher in the group treated with the hUC-MSC-CUR compared to those of other two groups. Therefore, the hUC-MSC-CUR may be a potential strategy to promote the proliferation and differentiation of PD cell model, therefore providing new insights into a novel therapeutic approach in PD.