Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity

Figure 3

Cell signaling pathway analysis in iE-DAP- and TNF-α-stimulated KN-3 cells. (a) KN-3 cells were treated with PD98059 (25 μM), SB203580 (10 μM), SP600125 (10 μM), or SN-50 (9 μM) for 1 h followed by stimulation with iE-DAP (10 μg mL⁻¹) or TNF-α (0.01 μg mL⁻¹) for 24 h. The concentrations of CINC-1, CINC-2, MCP-1, and CCL20 in cell culture supernatants were determined by ELISA. Values represent the means ± SDs from representative of three independent experiments and each experiment was performed in triplicate. Asterisks indicate significant differences ( and ) versus nonstimulated control group. (b) Immunoblotting analysis of IκBα in nonstimulated control and iE-DAP- or TNF-α-stimulated KN-3 cells. KN-3 cells were stimulated with iE-DAP (10 μg mL⁻¹), iE-Lys (10 μg mL⁻¹), or TNF-α (0.01 μg mL⁻¹) for 5 or 10 min. Equal loading of gels was confirmed with both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using an anti-actin antibody. The results shown are representative images of three independent experiments with similar results.

(a)

(b)