Prenatal Dexamethasone Exposure Increases the Susceptibility to Autoimmunity in Offspring Rats by Epigenetic Programing of Glucocorticoid Receptor

Table 1

Primers for real-time PCR.

Name	Accession number	Primers	Length (bp)	(°C)
GAPDH	NM_017008	F: 5′-GCAAGTTCAACGGCACAG-3′	140	60.5
		R: 5′-GCCAGTAGACTCCACGACAT-3′
GR	AJ271870	F: 5′-ACTGCTGGAGGTGATTGA-3′	157	53.2
		R: 5′-GTTTCTGAAGCCTGGTAT-3′
Exon 1-1	AJ271870	F: 5′-CCAGGAAGAAGTTTCAGAT-3′	195	57
		R: 5′-GATGCAGAAACCTTGACTGT-3′
Exon 1-4	AJ271870	F: 5′-CCCCAAAGCAACACCGTA-3′	186	56.1
		R: 5′-CAGGGACTTCGTCTCTACCAG-3′
Exon 1-5	AJ271870	F: 5′-GGCATGCAACTTCCTCCGAGTTTA-3′	176	55.4
		R: 5′-AGGGCGAAGATGCAGAAACCTTGA-3′
Exon 1-6	AJ271870	F: 5′-GGGCTCACATTAATATTTGC-3′	149	54.3
		R: 5′-AAACCTTGACTGTAGCTCCTC-3′
Exon 1-7	AJ271870	F: 5′-AAGAAACTCGGTTTCCCT-3′	167	55
		R: 5′-AACCTTGACTGTAGCTCCTC-3′
Exon 1-8	AJ271870	F: 5′-CGACAGTCGCCAACAGTTAAT-3′	81	57.3
		R: 5′-TGCCAGGGACTTCGTCTCTA-3′
Exon 1-9	AJ271870	F: 5′-CGGGGACGGATTCTAAT-3′	164	55.1
		R: 5′-AGATGCAGAAACCTTGACTGT-3′
Exon 1-10	AJ271870	F: 5′-CGCCGACTTGTTTATCTG-3′	200	53.7
		R: 5′-AACCTTGACTGTAGCTCCTC-3′
Exon 1-11	AJ271870	F: 5′-GCCGCAGAGAACTCAACAGT-3′	152	58
		R: 5′-GACTGTAGCTCCTCCCCTCA-3′