Research Article
Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System
Figure 1
Construction of plasmid pETduet-CK-T7 for the preparation of CK-T7 extract. The creatine kinase and T7 RNA polymerase sequences were cloned into MCS1 and MCS2 of pETduet-1, respectively.