Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System

Figure 4

Purification of the soluble recombinant SnRK2.1 expressed in cell-free system. Reaction mixture was centrifuged to separate soluble and insoluble fractions. Soluble SnRK2.1 was diluted with column buffer A and directly purified using Ni-NTA column. M, maker; Lane 1, diluted supernatant sample; Lane 2, flow through; Lane 3, washing with 20 mM imidazole; Lane 4, elution with 300 mM imidazole.