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BioMed Research International
Volume 2016, Article ID 9561058, 7 pages
Research Article

The Expression of Osteopontin and Wnt5a in Articular Cartilage of Patients with Knee Osteoarthritis and Its Correlation with Disease Severity

1Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
2Department of Orthopaedics, Affiliated Hospital, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
3Department of Joint Surgery, Changsha Central Hospital, Changsha, Hunan 410004, China

Received 7 March 2016; Revised 8 June 2016; Accepted 28 June 2016

Academic Editor: Przemko Tylzanowski

Copyright © 2016 Yusheng Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objectives. This study is undertaken to investigate the relation between osteopontin (OPN) and Wnt5a expression in the progression and pathogenesis of osteoarthritis (OA). Methods. 50 cartilage tissues from knee OA patients and normal controls were divided into four groups of severe, moderate, minor, and normal lesions based on the modified grading system of Mankin. Immunohistochemistry and real-time PCR were utilized to analyze the OPN and Wnt5a expression in articular cartilage. Besides, the relations between OPN and Wnt5a expression and the severity of OA were explored. Results. OPN and Wnt5a could be identified in four groups’ tissues. Amongst the groups, the intercomparisons of OPN expression levels showed statistical differences (). Besides, the intercomparisons of Wnt5a expression degrees showed statistical differences (), except that between the minor and normal groups (). The scores of Mankin were demonstrated to relate to OPN expression (, ) and Wnt5a expression in every group (, ). Also, a positive correlation can be observed between the OPN and Wnt5a expression (, ). Conclusion. In articular cartilage, the expressions of OPN and Wnt5a are positively related to progressive damage of knee OA joint. The correlation between Wnt5a and OPN might be important to the progression and pathogenesis of knee OA.