Study of Valproic Acid-Enhanced Hepatocyte Steatosis

Figure 1

OLA induces lipid accumulation in FL83B cells in a dose-dependent manner. Microscopic images of FL83B cells treated with BSA only (a), or 25 μM (b), 50 μM (c), 100 μM (d), 200 μM (e), and 400 μM (f) BSA-conjugated OLA for 24 hours. Cells were fixed and stained with Hoechst 33342 (blue, nucleus) and Nile Red (yellow, intracellular lipid droplets). Quantification of intracellular lipid accumulation was performed using a fluorescent microplate reader (g). Values are means ± SEM of four independent experiments. above the bars refer to significant differences (). CON: control, treated with normal culture medium only.

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