Study of Valproic Acid-Enhanced Hepatocyte Steatosis

Figure 4

VPA increases the mRNA and cell-surface protein expression levels of fatty acid translocase CD36. The mRNA expression levels of lipid transport-related genes including Cd36, Lrp, Ldlr, and Mttp were analyzed using real-time PCR (a). Relative fold changes were calculated using Ct values obtained from three independent experiments and are shown as means ± SEM. above the bars refer to significant differences (). The Fl83B cell-surface CD36 expression levels were analyzed by flow cytometry using mouse anti-CD36 antibody and DyLight 650-conjugated secondary antibody, and representative histograms (b) and quantitative flow cytometry data (c) are shown. above the bars refer to significant differences (). For background fluorescent subtraction, mouse isotype IgG antibody was used (OLA = 100 μM, VPA = 1 mM, 24 hours).

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