Review Article | Open Access
Zebrafish: A Versatile Animal Model for Fertility Research
The utilization of zebrafish in biomedical research is very common in the research world nowadays. Today, it has emerged as a favored vertebrate organism for the research in science of reproduction. There is a significant growth in amount numbers of scientific literature pertaining to research discoveries in reproductive sciences in zebrafish. It has implied the importance of zebrafish in this particular field of research. In essence, the current available literature has covered from the very specific brain region or neurons of zebrafish, which are responsible for reproductive regulation, until the gonadal level of the animal. The discoveries and findings have proven that this small animal is sharing a very close/similar reproductive system with mammals. More interestingly, the behavioral characteristics and along with the establishment of animal courtship behavior categorization in zebrafish have laid an even stronger foundation and firmer reason on the suitability of zebrafish utilization in research of reproductive sciences. In view of the immense importance of this small animal for the development of reproductive sciences, this review aimed at compiling and describing the proximate close similarity of reproductive regulation on zebrafish and human along with factors contributing to the infertility, showing its versatility and its potential usage for fertility research.
Danio rerio, or commonly known as zebrafish, is a tropical freshwater fish. It was previously a well-known aquarium fish at home, which has rapidly transformed into an indispensable animal model for scientists of today’s world. The numerous advantages and characteristics possessed by this small animal have never failed in tempting researchers in utilizing this animal model for their scientific research projects. Perhaps, the popular usage of this animal owns to their cheap and easy maintenance of animal in the laboratory [1, 2]. Nonetheless, the fact that well-characterized gene functions of zebrafish which is showing a high degree of similarity with human gene have certainly improved confidence level and potential implications of research findings [3, 4]. This may explain the drastic usage growth of this small animal in experimentation in recent years. Basically, the studies which have been done with zebrafish had basically contributed to a vast advancement in many fields of science. The usage of zebrafish in scientific research could be seen playing significant roles in fundamental research such as evolutionary science, genetics, neurobiology, and development biology [5–7]. In terms of application sciences, it has been widely utilized for drug discovery or even environmental monitoring effort [8–10].
Fertility or reproductive science is one of the popular fields in medical research. The impactful discoveries in this field are including the assisted reproductive technology (in vitro fertilization), erectile dysfunction medication such as cGMP-specific phosphodiesterase, and hormonal treatment techniques to overcome infertility [11, 12]. Unfortunately, infertility problems are still persisting around the globe with an alarming percentage of around 20% of infertile couples . This thus explained that the need of continuous research in fertility and further advancement in level of fundamental understanding of the reproductive system in human is a must in general.
In this context, zebrafish have swum into view as a promising model in assessing reproductive complications owing to its developmental and physiological advantages [14–17]. The short cycle of reproductive period and the transparency of these animals at early developmental stages are allowing the researchers to carry out research activities in more efficient or hassle-free way than before [18, 19]. A close degree of similarity of reproductive regulation systems between human and zebrafish has also permitted the researchers to study and understand the system in a more comprehensive way. This could be also seen from the identification of important neurons which are involved in regulating the reproductive system and presence of similar reproductive hormones and responses in this animal [20, 21]. Along with these fundamental research findings, zebrafish have indirectly granted the scientists evaluating the potential hazardous compounds on reproductive system on human. Furthermore, zebrafish are amenable to genetic manipulation which has offered another important aspect for researchers to study the gene effects on reproduction [22, 23]. Together with the establishment of courtship behavior in zebrafish [1, 24], it dispelled the pervasive myths of zebrafish usage in fertility research.
2. Reproductive Gender and Biology of Zebrafish
Mammals have dimorphic sex chromosomes and practice male heterogametic system. Gene SRY (sex determining region Y) is of large effect on mammals’ sex determination by acting as a genetic switch that initiates male pathway in bipotential gonad [25, 26]. Zebrafish, however, lack of the sex determination cascade. Complex sex determination system with combined effects of genetic and environmental factors such as surrounding temperature , exposure to sex hormones (e.g., oestrogen and androgen), and oxygen availability  have been revealed by consistent works in gonad ontogenetic differentiation of zebrafish. On the genetic point of view, recent studies have suggested chromosome 4 as the potential sex chromosome in natural zebrafish with their sex determination mechanism strongly weakened in domesticated zebrafish strain [29, 30].
On the other hand, similar to humans, several autosomal genes have proven significant roles in development and differentiation of gonads and reproductive cells. For instance, Anti-Müllerian Hormone (amh) is one of the critical hormones in sex differentiation during fetal development. Under tight transcriptional regulation by sox9, steroidogenic factor 1 (SF-1), Wilm’s tumor suppressor gene 1 (wt1), and GATA4, amh is released from the Sertoli cells in fetal testes [31–33]. In addition to degeneration of Müllerian ducts, a pair of ducts which further develops into Fallopian tubes and uterus, amh also inhibits the expression of a P450 aromatase enzyme, known as Cyp19a1, which converts androgens to estrogens . In this context, zebrafish share similar features of vertebrate gonadogenesis by having amh expression in their gonad along with the identification of gene binding sites for the same transcriptional factors in the amh gene promoter sequence [35, 36]. Besides, inhibition of early spermatogonial differentiation remains as the other known aspect of conserved bioactivity of amh between zebrafish and mammals .
Meanwhile, zebrafish have short generation time by having all of the precursors for major organs after 24 hours of fertilization and typically achieve reproductive maturity within 3 to 6 months after fertilization with the maturity period corresponding to the body length of approximately 23 mm . Although mice have similar development length, zebrafish which are oviparous can produce around 200 to 300 eggs per week, thus permitting large-scale experimental analysis. High level of genetic homology is also shared across both species . On the other hand, zebrafish display similar anatomy of germ cell organs to that in humans [38, 39]. Male zebrafish have paired testes with tubule organizations. Within each tubule, the walls are lined by Sertoli cells and they function mainly to support testes morphogenesis and spermatogenesis while Leydig cells detected in the interstitial spaces act as primary testosterone producer [38, 39]. One distinct spermatogenesis pattern observed in zebrafish is the presence of spermatogenic cyst which consists of a group of Sertoli cells enveloping germ cells that develop synchronously, instead of having few germ cells with different development stages in Sertoli cell as observed in higher vertebrates . On the other hand, study also showed the presence of accessory sperm duct gland in male zebrafish which functions mainly in the secretion of mucosubstances and production of sperm trails .
While for female zebrafish, the key similarities of the reproductive system lie in the structure and functions of ovaries. A pair of bilateral ovaries is observed in female and it is located between the swim bladder and abdominal wall . Ovarian wall is lined with thin epithelium with numbers of oogonia and oocyte follicles surrounded by interstitial tissues and somatic cells observed. Lobulated structures with interlobular spaces and the joining with oviduct have been revealed through histological sectioning . Across vertebrates, ovaries are the site of development and production of female gametes [44, 45]. There are four stages of ovarian development in zebrafish, namely, primary oocyte stage with observation of relatively small spherical cells, cortical-alveolar stage with enlarged oocytes filled with cortical alveoli, vitellogenic stage characterized by presence of egg yolk in oocytes, and finally maturation stage in which oocytes with irregular layer can be observed . Similar to other teleost fish and humans, zebrafish follicle contains an oocyte surrounded by zone radiata along with a follicular layer made up of inner granulosa cells and outer thecal cells layer . Ovulation takes place following rupture of the layers and it is mainly induced by male gonadal pheromones [24, 44]. It is also significantly promoted with the accumulation of steroid glucuronides such as 5a-androstane-3a, l7β-diol, and cholesterol in male holding water and administration of testes homogenates [38, 48].
Altogether, besides the biological advantages of zebrafish which include rapid embryonic development, large embryonic production, and high degree of similarity to human genome, there are striking homologies between the reproductive system of human and zebrafish and the many similarities in aspects spanning from the reproductive anatomy and physiology to gene functions and expression. As such, they serve as the ideal system for analyzing fertility as well as embryonic development.
2.1. Reproductive Behavior and Performance of Zebrafish
Zebrafish are early morning breeders and group spawners [24, 49]. Females proved capable of spawning at frequent but irregular basis, with several hundred of eggs in a spawning session . An interspawning frequency of approximately one to six days is observed . Eggs spawned by zebrafish are optically translucent and are normally larger as compared to other fishes, with approximately 0.7 mm in diameter . Besides having healthy sexual organ and morphological sexual characteristics development and undisrupted steroidogenesis [52, 53], normal courtship behavior is one of the crucial criteria for successful reproduction among zebrafish . Both male and female zebrafish display different mating behavior. The five typical behavior displayed by male zebrafish are chase in the form of swimming or following the females (chase), having contact with female by using its nose or tail (tail-nose), circling around females (encircle), circling around females in the “figure eight” pattern (zig-zag), and rapid tail movement against females’ bodies (quiver) [1, 24, 54]. While for females, their sexual behaviors begin with approach by swimming abruptly towards males (approach), swimming alongside males or staying still when being chased (escort), swimming around males or halting in front of males (present), and swimming to one preferred location in its habitat (lead) and oviposition (egg-lay) [1, 24].
During a courtship episode, chase, tail-nose, and approach are the three initiatory mating activities displayed by both genders of zebrafish followed by present and escort from females as receptive behavior [1, 24]. However, some females may chase males away aggressively when the male’s approach is unfavorable. Then, repetitive behaviors such as encircle and zig-zag are presented [1, 24]. After the display of repetitive behavior, female zebrafish start to swim towards a specific location for at least three times . Finally, males swim and spread their caudal and dorsal fins around females for alignment of their genital pores. Rapid tail oscillation can then be observed to encourage spawning [1, 49, 54]. Studies suggested the simultaneous release of sperms and eggs. To be precise, sperms are released before egg deposition . Generally, male courtship behavior peaks in the first 30 minutes of courtship period and it may continue for an hour . For both territorial and nonterritorial males, the same courtship behavior can be identified. However, nonterritorial males tend to pursue females in the whole available mating space whereas territorial males display their mating behavior limited to the areas close to spawning site and other males’ approaches are often unwelcomed .
Reproductive performance of zebrafish is affected by several environmental factors such as photoperiod [56, 57], tank volume , water temperature and pH , topography, fish densities, and presence of natural habitats items such as aquatic plants and substrates . Zebrafish have endogenous reproduction rhythm which is significantly influenced by photoperiod and a cycle of 10-hour light and 14-hour dark has been normally practiced for breeding [56, 57]. In both wild and laboratory environments, zebrafish normally spawn in the first few hours of daylight [1, 24]. However, spawning in the afternoon by wild zebrafish and in the late evening by zebrafish in captivity have also been observed . Additionally, they prefer to spawn in the areas with natural habitats items such as aquatic plants and substrates as well as in shallow areas with greater embryo production observed . On the other hand, chamber volume varies according to the number and size of breeding adults. A tank volume of not less than 300 mL is recommended for successful breeding between six zebrafish with weight ranges from 0.50 g to 0.70 g and 0.95 g for male and female, respectively . Meanwhile, zebrafish breeders normally go with a water temperature of 24 to 30°C along with pH between 7.0 and 8.0 . Feeding practices which include type of diet, frequency, and density of feeding are also of significant importance in zebrafish spawning. Several recommended diets for breeding zebrafish have been suggested. These include feeding zebrafish with formulated diet, Gemma Micro 300 at 5% of body weight once daily , flake diet to satiation three times daily or on a rotating diet of flake food and freshly hatched brine shrimp (Artemia nauplii) in every morning and evening, respectively [61, 62], and Spirulina platensis-based diet three times daily at 5% of body weight . Meanwhile, nutritional supplementation in phospholipids (phosphatidylcholine) , highly unsaturated fatty acids (e.g., diet with 1 : 1 squid oil : linseed oil)  and Moringa leaf  have been proved to promote reproductive system of zebrafish. It is important to note that breeding zebrafish require rich feeding.
Phenotypic cues such as paternal and maternal body size [51, 67, 68], fin length [69, 70], group size , and behavioral traits  have been extensively studied to identify their potential effects on the reproductive success of zebrafish. Besides the well-known fact that large females displayed higher fecundity along with provision of high qualities of eggs and larvae [51, 67], pronounced size-dependent paternal effect on a broad range of reproductive parameters is identified. Large (28-29 mm) and very large (30-31 mm) males can contribute to higher hatching probability along with early hatching time and larger offspring hatched [51, 68]. In an indirect male-size effect, females have shown their preference towards large (26–34 mm), territorial males by allocating more eggs to them as compared to small males [24, 51, 73]. Meanwhile, studies showed that wild type females do not show preferences towards long and short fin males, hence suggesting that it is the total body size that females prefer as compared to overall apparent size [69, 70]. However, one study discovered the strong association between long fin males and females . Besides visual information, adult females display mate selection in response to olfactory cue. They showed strong preference towards odour stimuli from nonkin males, thus avoiding inbreeding which often leads to reduced fecundity and quality of offspring . On the other hand, lower reproductive success in terms of mean per capita egg production was observed at higher fish densities (e.g., 5 males and 10 females), owing to increased aggression level among males and competition among females over oviposition site . On top of that, decreased courtship rate was shown in the high density male-biased group. This observation can be explained by the tendency of territorial males to engage in territorial defense, rather than in mate acquisition [24, 71]. In view of the significant impact of population density and sex ratio on mating success of zebrafish, a small mating group of approximately five along with male to female ratio of 1 : 2 is often recommended for effective breeding . During a courtship period, males often compete with each other. Besides acquiring a territory and maintaining dominancy, study illustrated that males that are bold and aggressive have greater reproductive fitness by allowing greater proportion of eggs fertilized .
3. Regulation of Reproductive System
The reproductive system is a functional cooperation among sex organs in an organism to produce a new life. In general, gametes producing gonads, ducts, and openings are some of the main reproductive elements shared among vertebrates . Normal sexual functioning requires strong genital muscles, extensive vascular network, and tight neuroendocrine regulations. In mammals, the reproductive system is tightly regulated by three interrelated hormonal feedback control axes: hypothalamic-pituitary-adrenal (HPA) axis, hypothalamic-pituitary-gonadal (HPG) axis, and hypothalamic-pituitary-thyroid (HPT) axis . The key components and functions of all of the three axes in zebrafish correspond closely to mammals .
3.1. Hypothalamic-Pituitary-Gonadal (HPG) Axis
Hypothalamic-Pituitary-Gonadal (HPG) axis is defined as a functional cooperation between three endocrine glands: hypothalamus, anterior pituitary gland (APG), and gonads in regulating reproduction, development, and aging in animals . In HPG axis, kisspeptin (Kiss1) neurons, and GnRH neurons are the two main control points in hypothalamus . Both of the neurons play important role in the regulation of the secretion of reproductive hormones, luteinizing hormone (LH), and follicular stimulating hormone (FSH) from APG [78, 79].
Evolutionary studies showed the presence of four Kiss-R genes lineages (Kiss-R1a, Kiss-R1b, Kiss-R2a, and Kiss-R2b). In humans, only Kiss-R1a lineage is conserved . Kiss1 neurons are the main mediator of sex steroid feedback loop [80, 81]. Along with this, sex steroid receptors such as estrogen receptor alpha receptors, androgen receptors, and progesterone receptors can be found on the neurons. As the neurons are colocalized with GnRH neurons, they are also defined as the upstream regulator of GnRH neurons in hypothalamus . In humans, there are more Kiss1 neurons in the arcuate (ARC) nucleus as compared to the anteroventral periventricular nucleus (AVPV) . Kiss1 neurons located in ARC act as the regulator in negative feedback mechanism of sex steroid hormones on the secretion of GnRH from GnRH neurons. While for Kiss1 neurons in AVPV, they mainly function in the preovulatory GnRH/LH surge process [82, 84].
To date, zebrafish remains as one of the few teleosts with detailed information gathered on the distribution and functions of kisspeptin. In zebrafish, two kiss genes, Kiss1 and Kiss2, have been identified successfully through in situ hybridization [85, 86]. Kiss1 neurons are limited to habenular nucleus while Kiss2 are widely distributed in ventral and caudal region of hypothalamus, thalamus, preoptic area, mesencephalon, and pallium . Meanwhile, few studies have reported the functional similarities of neuropeptide kisspeptin between zebrafish and mammals. Similar to mammalian Kiss1 signaling, habenular Kiss1 in zebrafish plays pivotal role in puberty onset through regulation of GnRH secretion [88, 89]. Additionally, regulation of gonadotropins release is also one of the potential physiological roles of kisspeptin in both zebrafish and human [83, 85]. Nevertheless, Kiss2 but not Kiss1 appears as the predominant GTH-I and GTH-II regulator in zebrafish .
GnRH neurons are neuron cells that play pivotal role in regulation of the release of reproductive hormones, LH and FSH from APG [79, 90]. Three types of GnRH genes: herring GnRH (GnRH1), chicken GnRH-II (cGnRH-II), and salmon GnRH (sGnRH/GnRH3) are identified in humans . GnRH1 is the classical hypothalamic reproductive neuroendocrine factor which further allows LH and FSH secretion from APG. In zebrafish, two forms of GnRH are identified, namely, chicken GnRH-II (cGnRH-II) in the midbrain tegmentum and salmon GnRH (sGnRH/GnRH3) which is expressed in olfactory bulb and preoptic area of hypothalamus [92, 93]. Unlike other fishes which possess three GnRH isoform , GnRH3, instead of GnRH1 takes the role of activating and controlling the pituitary release of LH and FSH in zebrafish .
In both humans and zebrafish, FSH (GTH-I in zebrafish) and LH (GTH-II in zebrafish) are produced by pituitary cells in response to GnRH from GnRH neurons in hypothalamus [83, 95]. The roles of these glycoprotein hormones in steroidogenesis and gametogenesis are conserved across species. In males, FSH regulates the spermatogonial proliferation and differentiation in Sertoli cells. While for females, FSH plays critical role in stimulating estrogen and inhibin alpha subunit (inha) production during folliculogenesis, as also reported in mammals [96, 97]. In mammals and zebrafish, expression of inha peaks during full-grown stage of follicles and it acts as an endocrine hormone which triggers final oocyte maturation and ovulation by stimulating LH production [98, 99]. In regard to the characterization of FSH receptors, zebrafish GTH-I receptors display strong sequence similarities to that of humans . Besides FSH, oocyte maturation and growth in both humans and zebrafish require the hormonal functions of LH and 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP), a maturation inducing hormone [101, 102]. Meanwhile, LH also regulates steroidogenesis in Leydig cells, though to a lesser extent in zebrafish . Nevertheless, cAMP/protein kinase A pathway remains as the common underlying mechanism in LH-mediated testicular steroid production across species . Viewing from the findings above, HPG axis of zebrafish appears to have striking resemblance to that of more evolved vertebrates, conserving the major outline of reproductive cells and hormones identified in mammals.
3.2. Hypothalamic-Pituitary-Thyroid (HPT) Axis
HPT is physiologically related to HPG and both of the axes work together in regulating reproductive functions . The presence of thyroid hormone receptors in ovaries and effect of estrogen hormone level on HPT axis have proven the reciprocal relationship between these two axes [75, 105]. In mammals, triiodothyronine (T3) and tetraiodothyronine (T4) are the two principal thyroid hormones secreted from the thyroid gland, a butterfly-shaped organ located in the neck. The hormonal output of thyroid gland is regulated by thyroid stimulating hormone (TSH) secreted from APG, which itself is controlled by thyroid releasing hormone (TRH) from hypothalamus . The main function of thyroid system is to regulate the metabolism, growth, and development of an individual. On the other hand, the amounts of thyroid hormones secreted are known to affect the release of reproductive hormones such as LH, FSH, and several steroid hormones, thus being consistent with the crosstalk concept between HPT and HPG [107–110] (Figure 1).
As the reproductive system is tightly regulated by HPT, several reproduction disorder symptoms will be displayed when the axis is disrupted [111–113]. With the excessive secretion of thyroid hormones from thyroid gland, or otherwise known as hyperthyroidism, women often experience menstrual disturbance and anovulatory cycles. In terms of menstrual disorder, amenorrhea and oligomenorrhea have been often reported, as well as a changes in the amount of menstrual flow such as hypomenorrhea and hypermenorrhea [114, 115]. On the other hand, semen quality is adversely affected in hyperthyroidism men [112, 116]. While for hypothyroidism, delayed puberty can be detected among teenagers and mature women tend to suffer from abnormal menstrual cycles and increased risk of fetal wastage [111, 113]. Following the decrease in the amount of LH, FSH, sex hormone binding protein, and serum testosterone level in hypothyroidism men, increased testicular size and decreased sperms qualities in terms of sperm morphology and motility and semen volume have been elucidated in several studies [117, 118].
In many aspects, thyroid system in teleost, particularly zebrafish, is similar to the mammalian system. Zebrafish apparently have thyroidal tissues with the same origin as those of mammals. Genes responsible for thyroid development such as pax2a and pax8 and nkx2.1a and hhex are conserved between zebrafish and mammals [119, 120]. Meanwhile, release of TSH from APG followed by synthesis of T3 from thyroid glands is observed in both organisms . The fundamental roles of thyroid hormones in regulating metabolism, early development, and differentiation of zebrafish correspond closely to thyroidal hormones functions in mammals. Additionally, alteration in the amount of thyroidal hormones secreted in zebrafish affects the regulation of reproduction system too (Figure 2). In male zebrafish, T3 stimulates mitotic activities in Sertoli cells as well as proliferation of type A undifferentiated spermatocytes . The recent studies have also shown that the change in concentrations of T3 and T4 may affect the levels of GTH-I and GTH-II, which are known to play important roles in stimulation of steroidogenesis and gametogenesis [52, 75]. Additionally, hyperthyroidism in larval zebrafish is shown to result in decreased aromatase activity along with estrogen synthesis, leading to testicular formation and skewed sex ratio in favor of males . The observed reproductive physiological changes under hyperthyroid condition are consistent across a range of animal models which includes mammals and reptiles, thus lending support to the statement regarding the masculinizing effect of thyroid hormones [123–125]. The combined potentiation effect of T3 and GTH-I on the androgen biosynthesis and sensitivity of testes further suggested the crosstalk between HPT and HPG in zebrafish . Altogether, the findings obtained on the components and reproductive functions of HPT axis reveal many parallel between zebrafish and human and this further delineates the remarkable potential of zebrafish as the animal model in infertility studies.
3.3. Hypothalamic-Pituitary-Adrenal (HPA) Axis
HPA axis is the complex set of interaction between three—hypothalamus, pituitary gland, and adrenal gland . It is the major constituent of neuroendocrine system which produces stress and mood responses and involves in the regulation of immune and reproductive system . Under stress condition, neuroendocrine neurons in the paraventricular nucleus of hypothalamus are stimulated to produce corticotrophin-releasing hormone (CRH) and vasopressin . These two hormone peptides in turn lead to the secretion of adrenocorticotropic (ACTH) hormones from APG. Biosynthesis of several corticosteroids such as cortisol can be observed following blood transportation of ACTH from APG to adrenal cortex . Cortisol is the steroid hormone produced from the zone fasciculate of the adrenal cortex in response to stress and low sugar level condition. Under stressful condition, the physiological demands for energy can be met through increased gluconeogenesis process stimulated by cortisol . At the meantime, cortisol prevents overactivation of immune system and inflammation during stress by allowing the shift towards type 2 helper T cells (Th2) immune response .
In zebrafish, stress axis is known as hypothalamus-pituitary-interrenal (HPI) axis . The anatomy and physiology of the pituitary are highly conserved between zebrafish and mammals. Similar to mammals, pituitary in zebrafish appears in two different parts with distinct functions. The hormones produced by pituitary glands under stress are the same as mammals [132, 133]. The pituitary-secreted stress hormones, ACTH, will then bind to type 2 melanocortin receptor (MC2R) located in the interrenal gland of zebrafish, the homolog of mammalian adrenal gland . Meanwhile, evolutionary conservation of MC2R trafficking and signaling was observed in zebrafish, particularly in terms of the presence of three forms of melanocortin 2 receptor accessory proteins (MRAP) and their structural features and the critical roles of MRAP 1 in MC2R signaling following ACTH stimulation and MRAP 1 or MRAP 2a in localization of MC2R to plasma membrane [135–137]. Across both species, cortisol is the main corticosteroid produced under stress condition. Cortisol stress signaling is primarily mediated by glucocorticoid receptor (GR), a ligand-activated transcription factor. In this context, studies suggested the presence of single GR gene with two splicing variants, termed GRα and GRβ in zebrafish, which shows high similarity level to its human equivalent [134, 138–140].
Infertility is defined as the incapability of an individual to achieve clinical pregnancy despite having regular unprotected sexual intercourse for more than 12 months. Epidemiology study showed that approximately 20% couples worldwide are suffering from infertility . In general, infertility is caused by male factors such as poor sperm qualities and quantities , female factors such as abnormal ovulation and tubal pathology [142, 143], combined male and female factors, and unexplained infertility factors . Hormonal imbalance, particularly due to unhealthy and stressful lifestyles [145, 146], and prolonged exposure to harmful chemicals and unfavorable environmental conditions [147–149] are some of the suggested underlying pathogenic mechanisms in infertility.
4.1. Stress-Induced Infertility
When zebrafish are exposed to stressor, nucleus preopticus (NPO), a region homologous to paraventricular nucleus (PVN) in hypothalamus of mammals, will secrete CRH. In response to CRH, corticotrophs in APG will release ACTH, the hormones which further stimulate cortisol biosynthesis in interrenal gland [132, 133]. The influence of HPI on reproductive axis in zebrafish is similar to that of mammals. The secretions of biological hormones such as CRF, ACTH, and cortisol under stress generally lead to impaired reproductive system through inhibition of the release of reproductive hormones and gametogenesis (Table 1) . In female zebrafish, the disruptive effects of ACTH and cortisol on gametogenesis and fertilization success have been illustrated through the identification of oocytes with DNA damage as well as reduced nucleic acid via disruption of protein synthesis . Additionally, ACTH induces strong vacuolization in zebrafish ooplasm and similar condition was also observed in mammalian adrenal gland cells following exposure to ACTH [151, 152]. On top of that, ACTH suppresses gonadotropin-stimulated estradiol release from ovarian follicles . This stress-induced inhibition of steroidogenesis may be related to the binding of ACTH to melanocortin 2 receptor (MC2R), a specific ACTH receptor identified in zebrafish ovary along with the presence of inhibitory G protein in MC2R signaling . To the best of our knowledge, currently there is no study identified on the effect of ACTH on male reproductive system. Nevertheless, MC2R receptors have been identified in male gonads and hence leading to the hypothesis that ACTH may involve in male gonadal steroid modulation too.
Following the high similarities identified in zebrafish HPG and HPA regulatory axis as compared to human, the reproductive health status of zebrafish under stress is highly predictive of mammalian responses and hence further strengthen the potential of zebrafish as research model in infertility studies (Figure 3).
4.2. Chemical-Induced Infertility
Since the beginning of industrial era in around 1750, a sharp increase in the amount of chemicals produced and released in the surrounding environment has been observed . At the same time, there is significant increase in the health threat following chemical exposure, leading to the increasing demand for robust and cost effective methods to assess the chemical effects in human health, particularly growth and development along with reproductive system [154, 155]. As aforementioned, mammals such as rat and mice have been normally used to assess the reproductive toxicity of chemicals. Unfortunately, mammals-based assays to assess reproductive toxicity are time-consuming, complex, and expensive to have large-scale experimental analysis . Moreover, high dosages are often required for mammal experimentation, thus leading to unpredictable toxicity levels of the environmental chemicals as the concentration levels of chemicals in the environment are often low . Hence, zebrafish are recommended as the model system in this research field, particularly for water-soluble pollutants following the ease of chemical introduction into zebrafish [158, 159] and increased throughput within a shorter research period (Figure 3).
4.2.1. Herbicide Residues (Glyphosate)
Focusing on glyphosate, or commercially known as Roundup, it is a chemical formulation in herbicide that has been used extensively in agricultural field worldwide and emerged in the topping list of herbicide usage in Western countries since 1974 . It controls the plants population by acting as an inhibitor for enzyme 5-enolpyruvylshikimate-3-phosphate synthase, an enzyme which catalyzes the production of intermediate in the plant biosynthesis of aromatic amino acids process . Although this biosynthesis pathway is absent in animals, studies have shown the reproductive adverse effects of glyphosate in a range of organisms, particularly aquatic organisms [162–164]. This water-soluble pollutant eventually affects human health, especially the sexual and reproductive development via consumption of contaminated food and drink [165–167].
Viewing the high structural similarities in the reproductive axis of zebrafish as compared to humans, zebrafish are often utilized as the model in the assessment of reproductive toxicity of environmental chemicals, including herbicides. Following exposure to high concentration of glyphosate, significant increase in expression of cyp19a1 gene, aromatase activity, and the predominant estrogen receptor in ovary, esr1, was identified, thus revealing the potential steroidogenesis disruption effect of glyphosate in zebrafish . It is hypothesized that the increased cyp19a1 and esr1 expression are compensatory mechanisms in ovary to restore the balance of estrogen hormone level . Similarly, a number of in vitro studies have revealed the potential of glyphosate as endocrine disruptor via inhibition of aromatase activities in human cell lines [168, 169]. The disruption of steroidogenic biosynthesis pathway was hypothesized as one of the major underlying factors which contributed to reduced egg productions along with histological evidence of ovarian follicle atresia in adult female zebrafish . Meanwhile, steroid hormone biosynthesis in testes was also affected. Upregulation of antioxidant genes and presence of sperms with lowered membrane and DNA integrity and motility were also observed in glyphosate-exposed adult male zebrafish, suggesting the potential of glyphosate in inducing oxidative stress in the testis . High parental exposure to glyphosate eventually caused increase in mortality rate of embryo during early development and this finding is generally in accordance with evidence from other species such as mammals  and amphibians .
4.2.2. Pesticides (Endosulfan)
Besides herbicide, aquatic environments are facing persistent pesticide pollution. A mixture of endosulfan I and II is often included in the pesticide formulation . Once it is released into the aquatic environment through field runoff and atmosphere transport, it exists in the form of endosulfan sulfate and diol in aquatic sediments and water, respectively [173, 174]. All these compounds are further broken down into alcohol, hydroxyl, ether, hydroxyl ether, and lactone . Endosulfan sulfate is the only toxic breakdown product and has longer half life up to years. Endosulfan is proven to be bioaccumulative and has potential effect on the reproductive performance, primarily via disruption of endocrine functions .
Ova-testes status, testicular damage, and sperms necrosis were observed among exposed adult male zebrafish at a very low concentration of endosulfan (10 ng/L) . The pathological changes in testes were highly correlated with the decreased hatching rate [176, 177]. Additionally, studies have proposed the binding ability of endosulfan to estradiol receptors found on liver, thus leading to increased vitellogenin level in male zebrafish . At the mean time, degenerative changes such as increased sizes of follicular cells, oocyte membrane folding, and reduced vitellogenesis can be observed on atretic follicles in female zebrafish . Besides, delayed sexual maturity and reduced spawning frequency were also observed . Altogether, reproductive toxicities of endosulfan, which include DNA damage and induction of oxidative stress [179, 180], developmental abnormalities , and histopathological changes of organs [182, 183], have been successfully illustrated by using zebrafish animal models.
4.2.3. 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a halogenated aromatic hydrocarbon compound and is normally released into the environment via organic synthesis and burning of organic materials . It is a potent developmental toxicant and endocrine disruptor . Studies have shown its reproductive toxicity manifested by altered gonad development , reduced egg production and survival rate of eggs and fry , and decreased serum estradiol and vitellogenin level .
Several estradiol-biosynthesis genes such as cyp19a1a, cyp11a1, and star have been pointed out as the potential gene suppression targets of TCDD . Meanwhile, downregulation of gonadotropin receptors and three estrogen receptors (esr1, esr2a, and esr2b) in the ovaries of adult zebrafish was observed . On the other hand, aryl hydrocarbon receptor (AHR) signaling cascade appears as one of the major gene suppression pathways induced by TCDD . The steroidogenesis disruption potential of TCDD is expressed by first binding to the AHR. The resulting AHR complex dimerizes with aromatic hydrocarbon receptor nuclear translocator (ARNT) protein in nucleus. Gene suppression in ovary is then observed following binding of the heterodimer complex to the aryl hydrocarbon-response element (AHRE) on genes, leading to disruption of estradiol biosynthesis in adult female ovaries . Depressed gonadotropin responsiveness and estradiol biosynthesis have resulted in damaged ovaries with retarded follicular maturation and ovarian functions, which further results in reduction of egg released and spawning activities [190, 191].
Despite the absence of testicular lesion in TCDD-exposed male zebrafish, males seem to have contributed more to TCDD-induced reproductive toxicity, which are mainly manifested by reduced number of eggs spawned and amount of fertilized eggs [185, 192]. On top of that, it is important to note that offspring from fish exposed to TCDD experience reduced reproductive capacity too . Conclusively, exposure to TCDD, especially during early life stages, brings adverse reproductive effects to both male and female zebrafish. It is important to note that the reproductive responses of zebrafish to TCDD are highly relevant to human following the high structural and functional similarities of the estrogen receptors in both zebrafish and humans and elucidation of perturbation of steroidogenesis regulation through AHR-dependent manner in TCDD-treated mammals [193–195].
4.2.4. Di(2-Ethylhexyl) Phthalate (DEHP)
Di(2-Ethylhexyl) phthalate (DEHP) is a commonly used plasticizer. Although it can be readily degraded by microorganism, continual releases of large chemical volume into atmosphere following plastic manufacture, burning activities, and waste water effluents have led to substantial concentration in aquatic system [196, 197]. Recently, its abilities to bind to estrogen receptor and contributions to reproductive toxicity in aquatic life and mammals have been discovered. Several studies were carried out on zebrafish to further evaluate the reproductive effect of DEHP [159, 198, 199].
In adult male zebrafish which received intraperitoneal injection of DEHP, impaired spermatogenesis with accumulation of spermatogonia in testes were observed [198, 199]. Additionally, DEHP is capable of inducing oxidative stress in testes with consequent increase in spermatozoa DNA fragmentation [198, 199]. Sharp decline in embryo production was also observed following the blockage of male hormone synthesis by DEHP. With the absence of male pheromones in water, female egg depositions followed by sperm release are inhibited . While for adult female zebrafish, impaired oocyte maturation and ovulation were the main toxicological effects of DEHP identified . Dose-related effects were observed on both of the defects with maturation signals from membrane progestin receptors β (mPRβ) and lhr greatly affected by low dose and ovulation signal from prostaglandin-endoperoxide synthase 2 (ptgs2) following high dose exposure . Increased circulating level of bone morphogenetic protein-15 (BMP15), a hormone regulator which prevents precocious oocyte maturation, was suggested as one of the factors which contributes to disrupted oocyte maturation . At the same time, suppressed expression of mPRβ following increased level of BMP15 contributed to the lack of egg production in DEHP-exposed zebrafish [159, 200].
4.2.5. Other Environmental Chemicals
In fact, zebrafish are increasingly used as powerful alternative model for assessing reproductive toxicity of a wide range of environmental chemicals as listed in Table 2. Most of the chemicals are industrial wastes and display high bioaccumulation factor. Disrupted gonad functions, altered steroidogenesis [75, 201, 202], and reduced quantities and qualities of germ cells along with low fertilization rate [201, 203, 204] are some of the reproductive toxicity effects observed in zebrafish chemical exposures (see Table 2).
As a whole, these findings provide strong rationales for conducting assessment on the reproductive toxicity of chemicals by using zebrafish. It was observed that most of the environmental chemicals disrupt the sexual functioning via perturbation of normal hormonal regulation of reproductive system. In view of the striking homologies in the endocrine regulation of reproduction as mentioned, there will be high relevance and predictability of chemical reproductive response between zebrafish and humans. The reproductive toxicity profile of the environmental chemicals established by using zebrafish animal model will be robust for uncovering the chemical-induced effects as well as appropriate protective approaches against chemical toxicity in human.
4.3. Environmental Induced Infertility
In the past few decades, effects of several environmental factors such as oxygen availability and exposure to exogenous heat on reproductive function have become of interest following the increase in the number of men who work in high altitude as well as in working areas with high heat exposure [205–207]. The reproductive effects of low oxygen availability in aquatic system, which are mainly due to eutrophication and organic pollution, have been extensively investigated in a range of fish, including zebrafish [28, 208–211]. Instead of causing direct cell damages in the reproductive organs, studies have discovered the negative indirect reproductive effect of hypoxia through alteration of circulating plasma sex steroid levels, notably testosterone and estradiol with underlying genetic and molecular mechanisms involving the expression of HPG-related genes , hypoxia-inducible factor 1 (HIF-1) [211, 212], cellular lipids and steroid hormones [210, 212], and leptin . Hormonal imbalance eventually leads to a lag in gonadal growth, masculinization of the ovary, sex ratio distortion in favor of males, and arrest in gametogenesis [28, 211]. Prominent reduction or complete absence of ovulating females observed under hypoxic condition correlates with both of the changes in steroid and contractile gene expression. On top of that, fertility defects caused by hypoxia are further implicated by aberrant primordial germ cell (PGC) migration . Collectively, despite the need for further elucidation of mechanisms underlying hypoxia-induced reproductive defect, the potential reproductive impairment of hypoxia in terms of abnormal gonadal development, reduced germ cell quantities and qualities, fertilization and hatching success, and larval and juvenile viability has been successfully revealed through the utilization of zebrafish as the animal model.
On the other hand, temperature of testicles is one of the critical factors which determines the sperms’ quality and quantity in humans and mammals [214, 215]. Testicular temperature of approximately 2 to 4°C lower than body temperature is required for normal testicular function. Basically, the temperature is regulated via two mechanisms: the dissipation of heat through the surface of scrotum and the heat lost from incoming arterial blood to outgoing venous blood . Through zebrafish study, anomalies in chromosomal number of the sperms were observed following the increase in water temperature . The germ cell aneuploidy is mainly due to mutation of monopolar spindle 1 (Mps1), the critical mitotic checkpoint kinase factor . As mentioned, sexual differentiation in some teleost can also be overridden by water temperature. Meanwhile, the influence of surrounding temperature on gonadal fate is also quite common among reptiles . Masculinizing effect of high water temperature often related to the induced oocyte apoptosis and differentiation of spermatogonia as well as suppressed activity of gonadal aromatase [27, 219]. Moreover, hatching rhythm is temperature-sensitive, with shorter hatching rate observed at constant water temperature of 28°C as compared to lower temperature of 24°C and thermocycles .
5. Overall Limitations
Zebrafish has only emerged over the past decade as a research model in reproductive field [79, 221]. As a result, there is a definite lack of detailed information on its reproductive system as compared to other well-developed higher vertebrate model organisms. For instance, there is still significant gap in our understanding of the underlying mechanisms in stress-mediated infertility, especially on male reproductive system . Additionally, more efforts are also needed to clarify the molecular genetic basis involved in zebrafish sex determination .
Additionally, zebrafish practice external insemination . One of the major shortcomings of this reproduction mode is the dilution of gametes concentration required for successful fertilization [223, 224]. However, zebrafish display evolutionary development in their mechanism of sperm releases. Instead of staying close to the females and directly releasing sperms into the water column as described in other fishes that display external insemination , sperm trails are first laid by male zebrafish onto the substrates’ surfaces . Mucosubstances secreted by seminal vesicles are probably acting as the adhesive material in which the sperms are embedded in . The production of sperm trails allows the release of active sperms over prolonged period of time, even after the males leave the spawning area, thus promoting egg insemination .
Despite the small size of zebrafish, the high similarities in reproductive functions and regulations between this small fish species and mammals have promoted them as the promising model in infertility research. Together with their biological advantages such as optical transparency during embryonic stage and rapid development, the utilization of zebrafish as model system has enabled us to delve more deeply and broadly into the reproductive functions. Additionally, our knowledge on the factors of infertility has been enriched by the researches of zebrafish. Human reproductive health risk assessment can thus be derived from the demonstration of underlying mechanisms associating infertility that are common between mammals and zebrafish. Most importantly, in-depth understanding about the underlying mechanisms leading to infertility contributes to the discovery and development of more effective fertility medications and technologies. Taken together, this review has highlighted the potential of zebrafish as valuable and reliable alternative model for studies aimed at answering questions concerning the reproductive functions as well as mechanisms of infertility in vertebrates.
The authors declare that there is no conflict of interests regarding the publication of this paper.
This work was supported by the Monash University Malaysia ECR Grant (5140077-000-00) and MOSTI eScience Fund (02-02-10-SF0215).
- K. O. Darrow and W. A. Harris, “Characterization and development of courtship in zebrafish, Danio rerio,” Zebrafish, vol. 1, no. 1, pp. 40–45, 2004.
- C. Zhao, X. Wang, Y. Zhao et al., “A novel xenograft model in zebrafish for high-resolution investigating dynamics of neovascularization in tumors,” PLoS ONE, vol. 6, no. 7, Article ID e21768, 2011.
- K. Howe, M. D. Clark, C. F. Torroja et al., “The zebrafish reference genome sequence and its relationship to the human genome,” Nature, vol. 496, no. 7446, pp. 498–503, 2013.
- E. E. Davis, S. Frangakis, and N. Katsanis, “Interpreting human genetic variation with in vivo zebrafish assays,” Biochimica et Biophysica Acta—Molecular Basis of Disease, vol. 1842, no. 10, pp. 1960–1970, 2014.
- R. E. Broughton, J. E. Milam, and B. A. Roe, “The complete sequence of the zebrafish (Danio rerio) mitochondrial genome and evolutionary patterns in vertebrate mitochondrial DNA,” Genome Research, vol. 11, no. 11, pp. 1958–1967, 2001.
- G. Golling, A. Amsterdam, Z. Sun et al., “Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development,” Nature Genetics, vol. 31, no. 2, pp. 135–140, 2002.
- G. A. Hortopan, M. T. Dinday, and S. C. Baraban, “Spontaneous seizures and altered gene expression in GABA signaling pathways in a mind bomb mutant zebrafish,” The Journal of Neuroscience, vol. 30, no. 41, pp. 13718–13728, 2010.
- H. Hollert, S. Keiter, N. König, M. Rudolf, M. Ulrich, and T. Braunbeck, “A new sediment contact assay to assess particle-bound pollutants using zebrafish (Danio rerio) embryos,” Journal of Soils and Sediments, vol. 3, no. 3, pp. 197–207, 2003.
- R. T. Peterson, S. Y. Shaw, T. A. Peterson et al., “Chemical suppression of a genetic mutation in a zebrafish model of aortic coarctation,” Nature Biotechnology, vol. 22, no. 5, pp. 595–599, 2004.
- A. V. Hallare, T. Kosmehl, T. Schulze, H. Hollert, H.-R. Köhler, and R. Triebskorn, “Assessing contamination levels of Laguna Lake sediments (Philippines) using a contact assay with zebrafish (Danio rerio) embryos,” Science of the Total Environment, vol. 347, no. 1–3, pp. 254–271, 2005.
- R. Homburg, A. Eshel, J. Kilborn, J. Adams, and H. S. Jacobs, “Combined luteinizing hormone releasing hormone analogue and exogenous gonadotrophins for the treatment of infertility associated with polycystic ovaries,” Human Reproduction, vol. 5, no. 1, pp. 32–35, 1990.
- D. S. Guzick, S. A. Carson, C. Coutifaris et al., “Efficacy of superovulation and intrauterine insemination in the treatment of infertility,” The New England Journal of Medicine, vol. 340, no. 3, pp. 177–183, 1999.
- P. Turchi, “Prevalence, definition, and classification of infertility,” in Clinical Management of Male Infertility, pp. 5–11, Springer, Berlin, Germany, 2015.
- O. Lee, A. Takesono, M. Tada, C. R. Tyler, and T. Kudoh, “Biosensor zebrafish provide new insights into potential health effects of environmental estrogens,” Environmental Health Perspectives, vol. 120, no. 7, pp. 990–996, 2012.
- J. Hou, L. Li, T. Xue, M. Long, Y. Su, and N. Wu, “Damage and recovery of the ovary in female zebrafish i.p.-injected with MC-LR,” Aquatic Toxicology, vol. 155, pp. 110–118, 2014.
- Z. Zhang, S.-W. Lau, L. Zhang, and W. Ge, “Disruption of zebrafish follicle-stimulating hormone receptor (fshr) but not luteinizing hormone receptor (lhcgr) gene by TALEN leads to failed follicle activation in females followed by sexual reversal to males,” Endocrinology, vol. 156, no. 10, pp. 3747–3762, 2015.
- I. Bassi, V. André, F. Marelli et al., “The zebrafish: an emerging animal model for investigating the hypothalamic regulation of reproduction,” Minerva Endocrinologica, vol. 41, no. 2, pp. 250–265, 2016.
- J.-H. He, J.-M. Gao, C.-J. Huang, and C.-Q. Li, “Zebrafish models for assessing developmental and reproductive toxicity,” Neurotoxicology and Teratology, vol. 42, pp. 35–42, 2014.
- A. Akhter, R. Kumagai, S. R. Roy et al., “Generation of transparent zebrafish with fluorescent ovaries: a living visible model for reproductive biology,” Zebrafish, vol. 13, no. 3, pp. 155–160, 2016.
- M. Laan, H. Richmond, C. He, and R. K. Campbell, “Zebrafish as a model for vertebrate reproduction: characterization of the first functional zebrafish (Danio rerio) gonadotropin receptor,” General and Comparative Endocrinology, vol. 125, no. 3, pp. 349–364, 2002.
- M. L. Blanton and J. L. Specker, “The hypothalamic-pituitary-thyroid (HPT) axis in fish and its role in fish development and reproduction,” Critical Reviews in Toxicology, vol. 37, no. 1-2, pp. 97–115, 2007.
- A. Rodríguez-Marí, C. Wilson, T. A. Titus et al., “Roles of brca2 (fancd1) in oocyte nuclear architecture, gametogenesis, gonad tumors, and genome stability in zebrafish,” PLoS Genetics, vol. 7, no. 3, Article ID e1001357, 2011.
- L. Chu, J. Li, Y. Liu, W. Hu, and C. H. K. Cheng, “Targeted gene disruption in zebrafish reveals noncanonical functions of LH signaling in reproduction,” Molecular Endocrinology, vol. 28, no. 11, pp. 1785–1795, 2014.
- R. Spence, G. Gerlach, C. Lawrence, and C. Smith, “The behaviour and ecology of the zebrafish, Danio rerio,” Biological Reviews, vol. 83, no. 1, pp. 13–34, 2008.
- K. Kashimada and P. Koopman, “Sry: the master switch in mammalian sex determination,” Development, vol. 137, no. 23, pp. 3921–3930, 2010.
- R. P. Piprek, “Molecular and cellular machinery of gonadal differentiation in mammals,” International Journal of Developmental Biology, vol. 54, no. 5, pp. 779–786, 2010.
- D. Uchida, M. Yamashita, T. Kitano, and T. Iguchi, “An aromatase inhibitor or high water temperature induce oocyte apoptosis and depletion of P450 aromatase activity in the gonads of genetic female zebrafish during sex-reversal,” Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, vol. 137, no. 1, pp. 11–20, 2004.
- E. H. H. Shang, R. M. K. Yu, and R. S. S. Wu, “Hypoxia affects sex differentiation and development leading to a male-dominated population in zebrafish (Danio rerio),” Environmental Science and Technology, vol. 40, no. 9, pp. 3118–3122, 2006.
- J. L. Anderson, A. Marí, I. Braasch et al., “Multiple sex-associated regions and a putative sex chromosome in zebrafish revealed by RAD mapping and population genomics,” PLoS ONE, vol. 7, no. 7, article e40701, 2012.
- C. A. Wilson, S. K. High, B. M. McCluskey et al., “Wild sex in zebrafish: loss of the natural sex determinant in domesticated strains,” Genetics, vol. 198, no. 3, pp. 1291–1308, 2014.
- P. De Santa Barbara, N. Bonneaud, B. Boizet et al., “Direct interaction of SRY-related protein SOX9 and steroidogenic factor 1 regulates transcription of the human anti-Müllerian hormone gene,” Molecular and Cellular Biology, vol. 18, no. 11, pp. 6653–6665, 1998.
- D. Lourenço, R. Brauner, M. Rybczyńska, C. Nihoul-Fékété, K. McElreavey, and A. Bashamboo, “Loss-of-function mutation in GATA4 causes anomalies of human testicular development,” Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 4, pp. 1597–1602, 2011.
- F. Barrionuevo and G. Scherer, “SOX E genes: SOX9 and SOX8 in mammalian testis development,” The International Journal of Biochemistry & Cell Biology, vol. 42, no. 3, pp. 433–436, 2010.
- A. Rodríguez-Marí, Y.-L. Yan, R. A. BreMiller, C. Wilson, C. Cañestro, and J. H. Postlethwait, “Characterization and expression pattern of zebrafish anti-Müllerian hormone (amh) relative to sox9a, sox9b, and cyp19a1a, during gonad development,” Gene Expression Patterns, vol. 5, no. 5, pp. 655–667, 2005.
- X. G. Wang and L. Orban, “Anti-Müllerian hormone and 11 β-hydroxylase show reciprocal expression to that of aromatase in the transforming gonad of zebrafish males,” Developmental Dynamics, vol. 236, no. 5, pp. 1329–1338, 2007.
- K. J. Groh, V. J. Nesatyy, H. Segner, R. I. L. Eggen, and M. J.-F. Suter, “Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio),” Fish Physiology and Biochemistry, vol. 37, no. 3, pp. 619–647, 2011.
- K. S. Skaar, R. H. Nóbrega, A. Magaraki, L. C. Olsen, R. W. Schulz, and R. Male, “Proteolytically activated, recombinant anti-Müllerian hormone inhibits androgen secretion, proliferation, and differentiation of spermatogonia in adult zebrafish testis organ cultures,” Endocrinology, vol. 152, no. 9, pp. 3527–3540, 2011.
- R. Van den Hurk and J. W. Resink, “Male reproductive system as sex pheromone producer in teleost fish,” Journal of Experimental Zoology, vol. 261, no. 2, pp. 204–213, 1992.
- K. R. Siegfried and C. Nüsslein-Volhard, “Germ line control of female sex determination in zebrafish,” Developmental Biology, vol. 324, no. 2, pp. 277–287, 2008.
- R. W. Schulz, R. H. Nóbrega, R. D. V. S. Morais, P. P. De Waal, L. R. França, and J. Bogerd, “Endocrine and paracrine regulation of zebrafish spermatogenesis: the Sertoli cell perspective,” Animal Reproduction, vol. 12, no. 1, pp. 81–87, 2015.
- J. R. Kemadjou Njiwa, P. Müller, and R. Klein, “Variations of sperm release in three batches of zebrafish,” Journal of Fish Biology, vol. 64, no. 2, pp. 475–482, 2004.
- T. Gupta and M. C. Mullins, “Dissection of organs from the adult zebrafish,” Journal of Visualized Experiments, no. 37, article e1717, 2010.
- A. L. Menke, J. M. Spitsbergen, A. P. M. Wolterbeek, and R. A. Woutersen, “Normal anatomy and histology of the adult zebrafish,” Toxicologic Pathology, vol. 39, no. 5, pp. 759–775, 2011.
- G. Gerlach, “Pheromonal regulation of reproductive success in female zebrafish: female suppression and male enhancement,” Animal Behaviour, vol. 72, no. 5, pp. 1119–1124, 2006.
- T. DeFalco and B. Capel, “Gonad morphogenesis in vertebrates: divergent means to a convergent end,” Annual Review of Cell and Developmental Biology, vol. 25, pp. 457–482, 2009.
- Ö. Çakıcı and S. İ. Üçüncü, “Oocyte development in the zebrafish, Danio rerio (Teleostei: Cyprinidae),” Journal of Fisheries & Aquatic Sciences, vol. 24, no. 1-2, pp. 137–141, 2007.
- E. Clelland and C. Peng, “Endocrine/paracrine control of zebrafish ovarian development,” Molecular and Cellular Endocrinology, vol. 312, no. 1-2, pp. 42–52, 2009.
- R. van den Hurk, W. G. E. J. Schoonen, G. A. van Zoelen, and J. G. D. Lambert, “The biosynthesis of steroid glucuronides in the testis of the zebrafish, Brachydanio rerio, and their pheromonal function as ovulation inducers,” General and Comparative Endocrinology, vol. 68, no. 2, pp. 179–188, 1987.
- S. Hutter, D. J. Penn, S. Magee, and S. M. Zala, “Reproductive behaviour of wild zebrafish (Danio rerio) in large tanks,” Behaviour, vol. 147, no. 5-6, pp. 641–660, 2010.
- A. Nasiadka and M. D. Clark, “Zebrafish breeding in the laboratory environment,” ILAR Journal, vol. 53, no. 2, pp. 161–168, 2012.
- S. Uusi-Heikkilä, A. Kuparinen, C. Wolter, T. Meinelt, and R. Arlinghaus, “Paternal body size affects reproductive success in laboratory-held zebrafish (Danio rerio),” Environmental Biology of Fishes, vol. 93, no. 4, pp. 461–474, 2012.
- X. Liu, K. Ji, A. Jo, H.-B. Moon, and K. Choi, “Effects of TDCPP or TPP on gene transcriptions and hormones of HPG axis, and their consequences on reproduction in adult zebrafish (Danio rerio),” Aquatic Toxicology, vol. 134-135, pp. 104–111, 2013.
- J. Deng, C. Liu, L. Yu, and B. Zhou, “Chronic exposure to environmental levels of tribromophenol impairs zebrafish reproduction,” Toxicology and Applied Pharmacology, vol. 243, no. 1, pp. 87–95, 2010.
- M. G. Larsen, K. B. Hansen, P. G. Henriksen, and E. Baatrup, “Male zebrafish (Danio rerio) courtship behaviour resists the feminising effects of 17α-ethinyloestradiol—morphological sexual characteristics do not,” Aquatic Toxicology, vol. 87, no. 4, pp. 234–244, 2008.
- R. Spence, W. C. Jordan, and C. Smith, “Genetic analysis of male reproductive success in relation to density in the zebrafish, Danio rerio,” Frontiers in Zoology, vol. 3, no. 1, article 5, 2006.
- B. Blanco-Vives and F. J. Sánchez-Vázquez, “Synchronisation to light and feeding time of circadian rhythms of spawning and locomotor activity in zebrafish,” Physiology and Behavior, vol. 98, no. 3, pp. 268–275, 2009.
- C. Lawrence, “The husbandry of zebrafish (Danio rerio): a review,” Aquaculture, vol. 269, no. 1–4, pp. 1–20, 2007.
- E. M. Goolish, R. Evans, K. Okutake, and R. Max, “Chamber volume requirements for reproduction of the zebrafish Danio rerio,” The Progressive Fish-Culturist, vol. 60, no. 2, pp. 127–132, 1998.
- A. K. Sessa, R. White, Y. Houvras et al., “The effect of a depth gradient on the mating behavior, oviposition site preference, and embryo production in the zebrafish, Danio rerio,” Zebrafish, vol. 5, no. 4, pp. 335–339, 2008.
- C. Lawrence, J. Best, A. James, and K. Maloney, “The effects of feeding frequency on growth and reproduction in zebrafish (Danio rerio),” Aquaculture, vol. 368-369, pp. 103–108, 2012.
- R. L. Hill Jr. and D. M. Janz, “Developmental estrogenic exposure in zebrafish (Danio rerio): I. Effects on sex ratio and breeding success,” Aquatic Toxicology, vol. 63, no. 4, pp. 417–429, 2003.
- M. L. Markovich, N. V. Rizzuto, and P. B. Brown, “Diet affects spawning in zebrafish,” Zebrafish, vol. 4, no. 1, pp. 69–74, 2007.
- B. Geffroy and O. Simon, “Effects of a Spirulina platensis-based diet on zebrafish female reproductive performance and larval survival rate,” Cybium, vol. 37, no. 1-2, pp. 31–38, 2013.
- P. Diogo, G. Martins, P. Gavaia et al., “Assessment of nutritional supplementation in phospholipids on the reproductive performance of zebrafish, Danio rerio (Hamilton, 1822),” Journal of Applied Ichthyology, vol. 31, supplement 1, pp. 3–9, 2015.
- A. Jaya-Ram, M.-K. Kuah, P.-S. Lim, S. Kolkovski, and A. C. Shu-Chien, “Influence of dietary HUFA levels on reproductive performance, tissue fatty acid profile and desaturase and elongase mRNAs expression in female zebrafish Danio rerio,” Aquaculture, vol. 277, no. 3-4, pp. 275–281, 2008.
- L. T. Paul, L. A. Fowler, R. J. Barry, and S. A. Watts, “Evaluation of Moringa oleifera as a dietary supplement on growth and reproductive performance in zebrafish,” Journal of Nutritional Ecology and Food Research, vol. 1, no. 4, pp. 322–328, 2013.
- S. Uusi-Heikkilä, C. Wolter, T. Meinelt, and R. Arlinghaus, “Size-dependent reproductive success of wild zebrafish Danio rerio in the laboratory,” Journal of Fish Biology, vol. 77, no. 3, pp. 552–569, 2010.
- S. Uusi-Heikkilä, L. Böckenhoff, C. Wolter, and R. Arlinghaus, “Differential allocation by female zebrafish (Danio rerio) to different-sized males—an example in a fish species lacking parental care,” PLoS ONE, vol. 7, no. 10, Article ID e48317, 2012.
- B. Kitevski and M. Pyron, “Female zebrafish (Danio rerio) do not prefer mutant longfin males,” Journal of Freshwater Ecology, vol. 18, no. 3, pp. 501–502, 2003.
- J. M. Gumm, J. L. Snekser, and M. K. Iovine, “Fin-mutant female zebrafish (Danio rerio) exhibit differences in association preferences for male fin length,” Behavioural Processes, vol. 80, no. 1, pp. 35–38, 2009.
- R. Spence and C. Smith, “Male territoriality mediates density and sex ratio effects on oviposition in the zebrafish, Danio rerio,” Animal Behaviour, vol. 69, no. 6, pp. 1317–1323, 2005.
- T. O. Ariyomo and P. J. Watt, “The effect of variation in boldness and aggressiveness on the reproductive success of zebrafish,” Animal Behaviour, vol. 83, no. 1, pp. 41–46, 2012.
- M. Pyron, “Female preferences and male-male interactions in zebrafish (Danio rerio),” Canadian Journal of Zoology, vol. 81, no. 1, pp. 122–125, 2003.
- G. Gerlach and N. Lysiak, “Kin recognition and inbreeding avoidance in zebrafish, Danio rerio, is based on phenotype matching,” Animal Behaviour, vol. 71, no. 6, pp. 1371–1377, 2006.
- C. Liu, X. Zhang, J. Deng et al., “Effects of prochloraz or propylthiouracil on the cross-talk between the HPG, HPA, and HPT axes in zebrafish,” Environmental Science and Technology, vol. 45, no. 2, pp. 769–775, 2011.
- M. Maalhagh, A. S. Jahromi, A. Yusefi et al., “Effects of prepubertal acute immobilization stress on serum kisspeptin level and testis histology in rats,” Pakistan Journal of Biological Sciences, vol. 19, no. 1, pp. 43–48, 2016.
- H. Vaudry and J. Y. Seong, “Neuropeptide GPCRs in neuroendocrinology,” Frontiers in Endocrinology, vol. 5, article 41, 2014.
- K.-C. Liu, S.-W. Lin, and W. Ge, “Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane,” Endocrinology, vol. 152, no. 11, pp. 4418–4430, 2011.
- R. Fontaine, P. Affaticati, K. Yamamoto et al., “Dopamine inhibits reproduction in female zebrafish (Danio rerio) via three pituitary D2 receptor subtypes,” Endocrinology, vol. 154, no. 2, pp. 807–818, 2013.
- S. Messager, E. E. Chatzidaki, D. Ma et al., “Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 5, pp. 1761–1766, 2005.
- X. D. De Tassigny and W. H. Colledge, “The role of Kisspeptin signaling in reproduction,” Physiology, vol. 25, no. 4, pp. 207–217, 2010.
- J. T. Smith, S. M. Popa, D. K. Clifton, G. E. Hoffman, and R. A. Steiner, “Kiss1 neurons in the forebrain as central processors for generating the preovulatory luteinizing hormone surge,” The Journal of Neuroscience, vol. 26, no. 25, pp. 6687–6694, 2006.
- M. N. Lehman, C. M. Merkley, L. M. Coolen, and R. L. Goodman, “Anatomy of the kisspeptin neural network in mammals,” Brain Research, vol. 1364, pp. 90–102, 2010.
- J. L. Robertson, D. K. Clifton, H. O. De La Iglesia, R. A. Steiner, and A. S. Kauffman, “Circadian regulation of Kiss1 neurons: implications for timing the preovulatory gonadotropin-releasing hormone/luteinizing hormone surge,” Endocrinology, vol. 150, no. 8, pp. 3664–3671, 2009.
- A. Servili, Y. Le Page, J. Leprince et al., “Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish,” Endocrinology, vol. 152, no. 4, pp. 1527–1540, 2011.
- S. Ogawa, K. W. Ng, P. N. Ramadasan, F. M. Nathan, and I. S. Parhar, “Habenular Kiss1 neurons modulate the serotonergic system in the brain of zebrafish,” Endocrinology, vol. 153, no. 5, pp. 2398–2407, 2012.
- Y. Song, X. Duan, J. Chen, W. Huang, Z. Zhu, and W. Hu, “The distribution of kisspeptin (Kiss)1- and Kiss2-positive neurones and their connections with gonadotrophin-releasing hormone-3 neurones in the zebrafish brain,” Journal of Neuroendocrinology, vol. 27, no. 3, pp. 198–211, 2015.
- T. Kitahashi, S. Ogawa, and I. S. Parhar, “Cloning and expression of kiss2 in the zebrafish and medaka,” Endocrinology, vol. 150, no. 2, pp. 821–831, 2009.
- S. J. Semaan, K. P. Tolson, and A. S. Kauffman, “The development of kisspeptin circuits in the Mammalian brain,” in Kisspeptin Signaling in Reproductive Biology, pp. 221–252, Springer, Berlin, Germany, 2013.
- I. R. Thompson and U. B. Kaiser, “GnRH pulse frequency-dependent differential regulation of LH and FSH gene expression,” Molecular and Cellular Endocrinology, vol. 385, no. 1-2, pp. 28–35, 2014.
- D. Yahalom, A. Chen, N. Ben-Aroya et al., “The gonadotropin-releasing hormone family of neuropeptides in the brain of human, bovine and rat: identification of a third isoform,” FEBS Letters, vol. 463, no. 3, pp. 289–294, 1999.
- A. Gopinath, L. A. Tseng, and K. E. Whitlock, “Temporal and spatial expression of gonadotropin releasing hormone (GnRH) in the brain of developing zebrafish (Danio rerio),” Gene Expression Patterns, vol. 4, no. 1, pp. 65–70, 2004.
- O. Palevitch, K. Kight, E. Abraham, S. Wray, Y. Zohar, and Y. Gothilf, “Ontogeny of the GnRH systems in zebrafish brain: in situ hybridization and promoter-reporter expression analyses in intact animals,” Cell and Tissue Research, vol. 327, no. 2, pp. 313–322, 2007.
- D.-K. Kim, E. B. Cho, M. J. Moon et al., “Revisiting the evolution of gonadotropin-releasing hormones and their receptors in vertebrates: secrets hidden in genomes,” General and Comparative Endocrinology, vol. 170, no. 1, pp. 68–78, 2011.
- M. Golan, E. Zelinger, Y. Zohar, and B. Levavi-Sivan, “Architecture of GnRH-gonadotrope-vasculature reveals a dual mode of gonadotropin regulation in fish,” Endocrinology, vol. 156, no. 11, pp. 4163–4173, 2015.
- C. M. Howles, “Role of LH and FSH in ovarian function,” Molecular and Cellular Endocrinology, vol. 161, no. 1-2, pp. 25–30, 2000.
- E. S. Clelland and S. P. Kelly, “Tight junction proteins in zebrafish ovarian follicles: stage specific mRNA abundance and response to 17β-estradiol, human chorionic gonadotropin, and maturation inducing hormone,” General and Comparative Endocrinology, vol. 168, no. 3, pp. 388–400, 2010.
- S.-K. Poon, W.-K. So, X. Yu, L. Liu, and W. Ge, “Characterization of inhibin α subunit (inha) in the zebrafish: evidence for a potential feedback loop between the pituitary and ovary,” Reproduction, vol. 138, no. 4, pp. 709–719, 2009.
- J. M. Guzmán, J. A. Luckenbach, Y. Yamamoto, and P. Swanson, “Expression profiles of Fsh-regulated ovarian genes during oogenesis in coho salmon,” PLoS ONE, vol. 9, no. 12, Article ID e114176, 2014.
- J. Bogerd, J. C. M. Granneman, R. W. Schulz, and H. F. Vischer, “Fish FSH receptors bind LH: how to make the human FSH receptor to be more fishy?” General and Comparative Endocrinology, vol. 142, no. 1-2, pp. 34–43, 2005.
- C. A. Lessman, “Oocyte maturation: converting the zebrafish oocyte to the fertilizable egg,” General and Comparative Endocrinology, vol. 161, no. 1, pp. 53–57, 2009.
- M. Golan, J. Biran, and B. Levavi-Sivan, “A novel model for development, organization, and function of gonadotropes in fish pituitary,” Frontiers in Endocrinology, vol. 5, article 182, 2014.
- Á. García-López, H. De Jonge, R. H. Nóbrega et al., “Studies in zebrafish reveal unusual cellular expression patterns of gonadotropin receptor messenger ribonucleic acids in the testis and unexpected functional differentiation of the gonadotropins,” Endocrinology, vol. 151, no. 5, pp. 2349–2360, 2010.
- A. G. Doufas and G. Mastorakos, “The hypothalamic-pituitary-thyroid axis and the female reproductive system,” Annals of the New York Academy of Sciences, vol. 900, pp. 65–76, 2000.
- M. T. Rae, O. Gubbay, A. Kostogiannou, D. Price, H. O. D. Critchley, and S. G. Hillier, “Thyroid hormone signaling in human ovarian surface epithelial cells,” The Journal of Clinical Endocrinology & Metabolism, vol. 92, no. 1, pp. 322–327, 2007.
- E. Bodó, B. Kany, E. Gáspár et al., “Thyroid-stimulating hormone, a novel, locally produced modulator of human epidermal functions, is regulated by thyrotropin-releasing hormone and thyroid hormones,” Endocrinology, vol. 151, no. 4, pp. 1633–1642, 2010.
- P. R. Manna, J. Kero, M. Tena-Sempere, P. Pakarinen, D. M. Stocco, and I. T. Huhtaniemi, “Assessment of mechanisms of thyroid hormone action in mouse Leydig cells: regulation of the steroidogenic acute regulatory protein, steroidogenesis, and luteinizing hormone receptor function,” Endocrinology, vol. 142, no. 1, pp. 319–331, 2001.
- A. Tohei, “Studies on the functional relationship between thyroid, adrenal and gonadal hormones,” Journal of Reproduction and Development, vol. 50, no. 1, pp. 9–20, 2004.
- L. C. Hall, E. P. Salazar, S. R. Kane, and N. Liu, “Effects of thyroid hormones on human breast cancer cell proliferation,” The Journal of Steroid Biochemistry and Molecular Biology, vol. 109, no. 1-2, pp. 57–66, 2008.
- E. Krajewska-Kulak and P. Sengupta, “Thyroid function in male infertility,” Frontiers in Endocrinology, vol. 4, article 174, 2013.
- K. Poppe, D. Glinoer, A. van Steirteghem et al., “Thyroid dysfunction and autoimmunity in infertile women,” Thyroid, vol. 12, no. 11, pp. 997–1001, 2002.
- E. Rijntjes, A. T. Wientjes, H. J. M. Swarts, D. G. De Rooij, and K. J. Teerds, “Dietary-induced hyperthyroidism marginally affects neonatal testicular development,” Journal of Andrology, vol. 29, no. 6, pp. 643–653, 2008.
- D. Unuane, H. Tournaye, B. Velkeniers, and K. Poppe, “Endocrine disorders & female infertility,” Best Practice & Research: Clinical Endocrinology & Metabolism, vol. 25, no. 6, pp. 861–873, 2011.
- J. V. Joshi, S. D. Bhandarkar, M. Chadha, D. Balaiah, and R. Shah, “Menstrual irregularities and lactation failure may precede thyroid dysfunction or goitre,” Journal of Postgraduate Medicine, vol. 39, no. 3, pp. 137–141, 1993.
- G. E. Krassas, N. Pontikides, Th. Kaltsas, Ph. Papadopoulu, and M. Batrinos, “Menstrual disturbances in thyrotoxicosis,” Clinical Endocrinology, vol. 40, no. 5, pp. 641–644, 1994.
- R. W. Hudson and A. L. Edwards, “Testicular function in hyperthyroidism,” Journal of Andrology, vol. 13, no. 2, pp. 117–124, 1992.
- J. J. C. Hernández, J. M. M. García, and L. C. García Diez, “Primary hypothyroidism and human spermatogenesis,” Systems Biology in Reproductive Medicine, vol. 25, no. 1, pp. 21–27, 1990.
- G. E. Krassas, F. Papadopoulou, K. Tziomalos, T. Zeginiadou, and N. Pontikides, “Hypothyroidism has an adverse effect on human spermatogenesis: a prospective, controlled study,” Thyroid, vol. 18, no. 12, pp. 1255–1259, 2008.
- P. Porazzi, D. Calebiro, F. Benato, N. Tiso, and L. Persani, “Thyroid gland development and function in the zebrafish model,” Molecular and Cellular Endocrinology, vol. 312, no. 1-2, pp. 14–23, 2009.
- T. Wendl, K. Lun, M. Mione et al., “Pax2.1 is required for the development of thyroid follicles in zebrafish,” Development, vol. 129, no. 15, pp. 3751–3760, 2002.
- F. Schmidt and T. Braunbeck, “Alterations along the hypothalamic-pituitary-thyroid axis of the zebrafish (Danio rerio) after exposure to propylthiouracil,” Journal of Thyroid Research, vol. 2011, Article ID 376243, 17 pages, 2011.
- S. Mukhi, L. Torres, and R. Patiño, “Effects of larval-juvenile treatment with perchlorate and co-treatment with thyroxine on zebrafish sex ratios,” General and Comparative Endocrinology, vol. 150, no. 3, pp. 486–494, 2007.
- N. K. Arambepola, D. Bunick, and P. S. Cooke, “Thyroid hormone effects on androgen receptor messenger RNA expression in rat Sertoli and peritubular cells,” Journal of Endocrinology, vol. 156, no. 1, pp. 43–50, 1998.
- A. Cardone, F. Angelini, T. Esposito, R. Comitato, and B. Varriale, “The expression of androgen receptor messenger RNA is regulated by tri-iodothyronine in lizard testis,” Journal of Steroid Biochemistry and Molecular Biology, vol. 72, no. 3-4, pp. 133–141, 2000.
- S. M. L. C. Mendis-Handagama and H. B. S. Ariyaratne, “Leydig cells, thyroid hormones and steroidogenesis,” Indian Journal of Experimental Biology, vol. 43, no. 11, pp. 939–962, 2005.
- R. D. V. S. Morais, R. H. Nóbrega, N. E. Gómez-González et al., “Thyroid hormone stimulates the proliferation of Sertoli cells and single type A spermatogonia in adult zebrafish (Danio rerio) testis,” Endocrinology, vol. 154, no. 11, pp. 4365–4376, 2013.
- M. Z. Khan, L. He, and X. Zhuang, “The emerging role of GPR50 receptor in brain,” Biomedicine & Pharmacotherapy, vol. 78, pp. 121–128, 2016.
- E. A. Mead and D. K. Sarkar, “Fetal alcohol spectrum disorders and their transmission through genetic and epigenetic mechanisms,” Frontiers in Genetics, vol. 5, article 154, 2014.
- C. Tsigos and G. P. Chrousos, “Hypothalamic-pituitary-adrenal axis, neuroendocrine factors and stress,” Journal of Psychosomatic Research, vol. 53, no. 4, pp. 865–871, 2002.
- J. Mouthaan, M. Sijbrandij, J. S. K. Luitse, J. C. Goslings, B. P. R. Gersons, and M. Olff, “The role of acute cortisol and DHEAS in predicting acute and chronic PTSD symptoms,” Psychoneuroendocrinology, vol. 45, pp. 179–186, 2014.
- M. L. M. Fuzzen, G. Van Der Kraak, and N. J. Bernier, “Stirring up new ideas about the regulation of the hypothalamic-pituitary- interrenal axis in zebrafish (Danio rerio),” Zebrafish, vol. 7, no. 4, pp. 349–358, 2010.
- D. Alsop and M. Vijayan, “The zebrafish stress axis: molecular fallout from the teleost-specific genome duplication event,” General and Comparative Endocrinology, vol. 161, no. 1, pp. 62–66, 2009.
- D. Alsop and M. M. Vijayan, “Molecular programming of the corticosteroid stress axis during zebrafish development,” Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, vol. 153, no. 1, pp. 49–54, 2009.
- D. Alsop and M. M. Vijayan, “Development of the corticosteroid stress axis and receptor expression in zebrafish,” American Journal of Physiology—Regulatory Integrative and Comparative Physiology, vol. 294, no. 3, pp. R711–R719, 2008.
- L. F. Chan, T. R. Webb, T.-T. Chung et al., “MRAP and MRAP2 are bidirectional regulators of the melanocortin receptor family,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 15, pp. 6146–6151, 2009.
- P. M. Hinkle and J. A. Sebag, “Structure and function of the melanocortin2 receptor accessory protein (MRAP),” Molecular and Cellular Endocrinology, vol. 300, no. 1-2, pp. 25–31, 2009.
- M. J. Agulleiro, S. Roy, E. Sánchez, S. Puchol, N. Gallo-Payet, and J. M. Cerdá-Reverter, “Role of melanocortin receptor accessory proteins in the function of zebrafish melanocortin receptor type 2,” Molecular and Cellular Endocrinology, vol. 320, no. 1-2, pp. 145–152, 2010.
- M. J. M. Schaaf, D. Champagne, I. H. C. Van Laanen et al., “Discovery of a functional glucocorticoid receptor β-isoform in zebrafish,” Endocrinology, vol. 149, no. 4, pp. 1591–1598, 2008.
- S. A. Cruz, C.-H. Lin, P.-L. Chao, and P.-P. Hwang, “Glucocorticoid receptor, but not mineralocorticoid receptor, mediates cortisol regulation of epidermal ionocyte development and ion transport in zebrafish (Danio rerio),” PLoS ONE, vol. 8, no. 10, Article ID e77997, 2013.
- A. Chatzopoulou, U. Roy, A. H. Meijer, A. Alia, H. P. Spaink, and M. J. M. Schaaf, “Transcriptional and metabolic effects of glucocorticoid receptor α and β signaling in zebrafish,” Endocrinology, vol. 156, no. 5, pp. 1757–1769, 2015.
- R. A. Saleh, A. Agarwal, E. A. Nada et al., “Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility,” Fertility and Sterility, vol. 79, no. 3, pp. 1597–1605, 2003.
- F. R. Parikh, S. G. Nadkarni, S. A. Kamat, N. Naik, S. B. Soonawala, and R. M. Parikh, “Genital tuberculosis—a major pelvic factor causing infertility in Indian women,” Fertility and Sterility, vol. 67, no. 3, pp. 497–500, 1997.
- J. S. E. Laven, B. Imani, M. J. C. Eijkemans, and B. C. J. M. Fauser, “New approach to polycystic ovary syndrome and other forms of anovulatory infertility,” Obstetrical & Gynecological Survey, vol. 57, no. 11, pp. 755–767, 2002.
- M. J. Jasper, K. P. Tremellen, and S. A. Robertson, “Primary unexplained infertility is associated with reduced expression of the T-regulatory cell transcription factor Foxp3 in endometrial tissue,” Molecular Human Reproduction, vol. 12, no. 5, pp. 301–308, 2006.
- V. R. Chidrawar, H. R. Chitme, K. N. Patel et al., “Effects of Cynodon dactylon on stress-induced infertility in male rats,” Journal of Young Pharmacists, vol. 3, no. 1, pp. 26–35, 2011.
- C. D. Lynch, R. Sundaram, J. M. Maisog, A. M. Sweeney, and G. M. Buck Louis, “Preconception stress increases the risk of infertility: results from a couple-based prospective cohort study—the LIFE study,” Human Reproduction, vol. 29, no. 5, pp. 1067–1075, 2014.
- C. A. Snijder, E. Te velde, N. Roeleveld, and A. Burdorf, “Occupational exposure to chemical substances and time to pregnancy: a systematic review,” Human Reproduction Update, vol. 18, no. 3, Article ID dms005, pp. 284–300, 2012.
- A. Zafar, S. A. M. A. S. Eqani, N. Bostan et al., “Toxic metals signature in the human seminal plasma of Pakistani population and their potential role in male infertility,” Environmental Geochemistry and Health, vol. 37, no. 3, pp. 515–527, 2015.
- M. Al-Griw, S. A. Al-Azreg, S. A. Al-Azreg et al., “Fertility and reproductive outcome in mice following Trichloroethane (TCE) exposure,” American Journal of Life Science Researches, vol. 3, no. 4, pp. 293–303, 2015.
- D. Alsop, J. S. Ings, and M. M. Vijayan, “Adrenocorticotropic hormone suppresses gonadotropin-stimulated estradiol release from zebrafish ovarian follicles,” PLoS ONE, vol. 4, no. 7, Article ID e6463, 2009.
- M. L. Sousa, F. Figueiredo, C. Pinheiro et al., “Morphological and molecular effects of cortisol and ACTH on zebrafish stage I and II Follicles,” Reproduction, vol. 150, no. 5, pp. 429–436, 2015.
- K. Volkova, N. Reyhanian Caspillo, T. Porseryd, S. Hallgren, P. Dinnétz, and I. Porsch-Hällström, “Developmental exposure of zebrafish (Danio rerio) to 17α-ethinylestradiol affects non-reproductive behavior and fertility as adults, and increases anxiety in unexposed progeny,” Hormones and Behavior, vol. 73, pp. 30–38, 2015.
- F. X. Han, A. Banin, Y. Su et al., “Industrial age anthropogenic inputs of heavy metals into the pedosphere,” Naturwissenschaften, vol. 89, no. 11, pp. 497–504, 2002.
- W. Wuttke, H. Jarry, and D. Seidlova-Wuttke, “Definition, classification and mechanism of action of endocrine disrupting chemicals,” Hormones, vol. 9, no. 1, pp. 9–15, 2010.
- A. Fucic, M. Gamulin, Z. Ferencic et al., “Environmental exposure to xenoestrogens and oestrogen related cancers: reproductive system, breast, lung, kidney, pancreas, and brain,” Environmental Health, vol. 11, supplement 1, article S8, 2012.
- T. Arora, A. Mehta, V. Joshi et al., “Substitute of animals in drug research: an approach towards fulfillment of 4R's,” Indian Journal of Pharmaceutical Sciences, vol. 73, no. 1, pp. 1–6, 2011.
- J. P. Myers, R. T. Zoeller, and F. S. vom Saal, “A clash of old and new scientific concepts in toxicity, with important implications for public health,” Environmental Health Perspectives, vol. 117, no. 11, pp. 1652–1655, 2009.
- C. Parng, W. L. Seng, C. Semino, and P. McGrath, “Zebrafish: a preclinical model for drug screening,” Assay and Drug Development Technologies, vol. 1, no. 1, pp. 41–48, 2002.
- O. Carnevali, L. Tosti, C. Speciale, C. Peng, Y. Zhu, and F. Maradonna, “DEHP impairs zebrafish reproduction by affecting critical factors in oogenesis,” PLoS ONE, vol. 5, no. 4, Article ID e10201, 2010.
- G. R. Heck, C. A. CaJacob, and S. R. Padgette, “Discovery, development, and commercialization of Roundup Ready® crops,” in Plant Biotechnology 2002 and Beyond, pp. 139–142, Springer, Berlin, Germany, 2003.
- F. M. Lopes, A. S. Varela Junior, C. D. Corcini et al., “Effect of glyphosate on the sperm quality of zebrafish Danio rerio,” Aquatic Toxicology, vol. 155, pp. 322–326, 2014.
- A. B. Soso, L. J. G. Barcellos, M. J. Ranzani-Paiva et al., “Chronic exposure to sub-lethal concentration of a glyphosate-based herbicide alters hormone profiles and affects reproduction of female Jundiá (Rhamdia quelen),” Environmental Toxicology and Pharmacology, vol. 23, no. 3, pp. 308–313, 2007.
- B. K. Dutra, F. A. Fernandes, D. M. Failace, and G. T. Oliveira, “Effect of roundup® (glyphosate formulation) in the energy metabolism and reproductive traits of Hyalella castroi (Crustacea, Amphipoda, Dogielinotidae),” Ecotoxicology, vol. 20, no. 1, pp. 255–263, 2011.
- T. M. Uren Webster, L. V. Laing, H. Florance, and E. M. Santos, “Effects of glyphosate and its formulation, roundup, on reproduction in zebrafish (Danio rerio),” Environmental Science & Technology, vol. 48, no. 2, pp. 1271–1279, 2014.
- C. Gasnier, C. Dumont, N. Benachour, E. Clair, M.-C. Chagnon, and G.-E. Séralini, “Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines,” Toxicology, vol. 262, no. 3, pp. 184–191, 2009.
- R. M. Romano, M. A. Romano, M. M. Bernardi, P. V. Furtado, and C. A. Oliveira, “Prepubertal exposure to commercial formulation of the herbicide glyphosate alters testosterone levels and testicular morphology,” Archives of Toxicology, vol. 84, no. 4, pp. 309–317, 2010.
- M. A. Romano, R. M. Romano, L. D. Santos et al., “Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression,” Archives of Toxicology, vol. 86, no. 4, pp. 663–673, 2012.
- S. Richard, S. Moslemi, H. Sipahutar, N. Benachour, and G.-E. Seralini, “Differential effects of glyphosate and roundup on human placental cells and aromatase,” Environmental Health Perspectives, vol. 113, no. 6, pp. 716–720, 2005.
- N. Benachour, H. Sipahutar, S. Moslemi, C. Gasnier, C. Travert, and G. E. Séralini, “Time- and dose-dependent effects of roundup on human embryonic and placental cells,” Archives of Environmental Contamination and Toxicology, vol. 53, no. 1, pp. 126–133, 2007.
- B. Winnick and E. M. Dzialowski, “The effects of glyphosate based herbicides on chick embryo morphology during development,” The Federal of American Societiesfor Experimental Biology Journal, vol. 27, p. 874.12, 2013.
- R. A. Relyea, “Growth and survival of five amphibian species exposed to combinations of pesticides,” Environmental Toxicology and Chemistry, vol. 23, no. 7, pp. 1737–1742, 2004.
- B.-H. Hwang and M.-R. Lee, “Solid-phase microextraction for organochlorine pesticide residues analysis in Chinese herbal formulations,” Journal of Chromatography A, vol. 898, no. 2, pp. 245–256, 2000.
- M. E. DeLorenzo, L. A. Taylor, S. A. Lund, P. L. Pennington, E. D. Strozier, and M. H. Fulton, “Toxicity and bioconcentration potential of the agricultural pesticide endosulfan in phytoplankton and zooplankton,” Archives of Environmental Contamination and Toxicology, vol. 42, no. 2, pp. 173–181, 2002.
- J. Weber, C. J. Halsall, D. Muir et al., “Endosulfan, a global pesticide: a review of its fate in the environment and occurrence in the Arctic,” Science of the Total Environment, vol. 408, no. 15, pp. 2966–2984, 2010.
- K. A. Stanley, L. R. Curtis, S. L. Massey Simonich, and R. L. Tanguay, “Endosulfan I and endosulfan sulfate disrupts zebrafish embryonic development,” Aquatic Toxicology, vol. 95, no. 4, pp. 355–361, 2009.
- Z. Han, S. Jiao, D. Kong, Z. Shan, and X. Zhang, “Effects of β-endosulfan on the growth and reproduction of zebrafish (Danio rerio),” Environmental Toxicology and Chemistry, vol. 30, no. 11, pp. 2525–2531, 2011.
- W. S. Chow, W. K.-L. Chan, and K. M. Chan, “Toxicity assessment and vitellogenin expression in zebrafish (Danio rerio) embryos and larvae acutely exposed to bisphenol A, endosulfan, heptachlor, methoxychlor and tetrabromobisphenol A,” Journal of Applied Toxicology, vol. 33, no. 7, pp. 670–678, 2013.
- A. Balasubramani and T. J. Pandian, “Endosulfan suppresses growth and reproduction in zebrafish,” Current Science, vol. 94, no. 7, pp. 883–890, 2008.
- B. Shao, L. Zhu, M. Dong et al., “DNA damage and oxidative stress induced by endosulfan exposure in zebrafish (Danio rerio),” Ecotoxicology, vol. 21, no. 5, pp. 1533–1540, 2012.
- M. Dong, L. Zhu, B. Shao et al., “The effects of endosulfan on cytochrome P450 enzymes and glutathione S-transferases in zebrafish (Danio rerio) livers,” Ecotoxicology and Environmental Safety, vol. 92, pp. 1–9, 2013.
- Y. M. Velasco-Santamaría, R. D. Handy, and K. A. Sloman, “Endosulfan affects health variables in adult zebrafish (Danio rerio) and induces alterations in larvae development,” Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, vol. 153, no. 4, pp. 372–380, 2011.
- M. C. F. Toledo and C. M. Jonsson, “Bioaccumulation and elimination of endosulfan in zebra fish (Brachydanio rerio),” Pesticide Science, vol. 36, no. 3, pp. 207–211, 1992.
- C. M. Jonsson and M. C. F. Toledo, “Acute toxicity of endosulfan to the fish Hyphessobrycon bifasciatus and Brachydanio rerio,” Archives of Environmental Contamination and Toxicology, vol. 24, no. 2, pp. 151–155, 1993.
- A. Sechman, P. Antos, D. Katarzyńska, A. Grzegorzewska, D. Wojtysiak, and A. Hrabia, “Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on secretion of steroids and STAR, HSD3B and CYP19A1 mRNA expression in chicken ovarian follicles,” Toxicology Letters, vol. 225, no. 2, pp. 264–274, 2014.
- T. C. K. Heiden, J. Spitsbergen, W. Heideman, and R. E. Peterson, “Persistent adverse effects on health and reproduction caused by exposure of zebrafish to 2,3,7,8-tetrachlorodibenzo-p-dioxin during early development and gonad differentiation,” Toxicological Sciences, vol. 109, no. 1, pp. 75–87, 2009.
- W. Z. Wu, W. Li, Y. Xu, and J. W. Wang, “Long-term toxic impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the reproduction, sexual differentiation, and development of different life stages of Gobiocypris rarus and Daphnia magna,” Ecotoxicology and Environmental Safety, vol. 48, no. 3, pp. 293–300, 2001.
- J. P. Giesy, P. D. Jones, K. Kannan, J. L. Newsted, D. E. Tillitt, and L. L. Williams, “Effects of chronic dietary exposure to environmentally relevant concentrations to 2,3,7,8-tetrachlorodibenzo-p-dioxin on survival, growth, reproduction and biochemical responses of female rainbow trout (Oncorhynchus mykiss),” Aquatic Toxicology, vol. 59, no. 1-2, pp. 35–53, 2002.
- T. K. Heiden, M. J. Carvan, and R. J. Hutz, “Inhibition of follicular development, vitellogenesis, and serum 17β-estradiol concentrations in zebrafish following chronic, sublethal dietary exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin,” Toxicological Sciences, vol. 90, no. 2, pp. 490–499, 2006.
- T. C. K. Heiden, C. A. Struble, M. L. Rise, M. J. Hessner, R. J. Hutz, and M. J. Carvan III, “Molecular targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) within the zebrafish ovary: insights into TCDD-induced endocrine disruption and reproductive toxicity,” Reproductive Toxicology, vol. 25, no. 1, pp. 47–57, 2008.
- R. Wannemacher, A. Rebstock, E. Kulzer, D. Schrenk, and K. W. Bock, “Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on reproduction and oogenesis in zebrafish (Brachydanio rerio),” Chemosphere, vol. 24, no. 9, pp. 1361–1368, 1992.
- T. K. Heiden, R. J. Hutz, and M. J. Carvan, “Accumulation, tissue distribution, and maternal transfer of dietary 2,3,7,8,-tetrachlorodibenzo-p-dioxin: impacts on reproductive success of zebrafish,” Toxicological Sciences, vol. 87, no. 2, pp. 497–507, 2005.
- T. R. Baker, R. E. Peterson, and W. Heideman, “Early dioxin exposure causes toxic effects in adult zebrafish,” Toxicological Sciences, vol. 135, no. 1, pp. 241–250, 2013.
- A. K. Dasmahapatra, B. A. B. Wimpee, A. L. Trewin, C. F. Wimpee, J. K. Ghorai, and R. J. Hutz, “Demonstration of 2,3,7,8-tetrachlorodibenzo-p-dioxin attenuation of P450 steroidogenic enzyme mRNAs in rat granulosa cell in vitro by competitive reverse transcriptase-polymerase chain reaction assay,” Molecular and Cellular Endocrinology, vol. 164, no. 1-2, pp. 5–18, 2000.
- N. H. Fukuzawa, S. Ohsako, Q. Wu et al., “Testicular cytochrome P450scc and LHR as possible targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the mouse,” Molecular and Cellular Endocrinology, vol. 221, no. 1-2, pp. 87–96, 2004.
- E. G. Notch and G. D. Mayer, “Efficacy of pharmacological estrogen receptor antagonists in blocking activation of zebrafish estrogen receptors,” General and Comparative Endocrinology, vol. 173, no. 1, pp. 183–189, 2011.
- S. Magdouli, R. Daghrir, S. K. Brar, P. Drogui, and R. D. Tyagi, “Di 2-ethylhexylphtalate in the aquatic and terrestrial environment: a critical review,” Journal of Environmental Management, vol. 127, pp. 36–49, 2013.
- Y. Zhan, J. Sun, Y. Luo et al., “Estimating emissions and environmental fate of di-(2-ethylhexyl) phthalate in Yangtze River Delta, China: application of inverse modeling,” Environmental Science & Technology, vol. 50, no. 5, pp. 2450–2458, 2016.
- T. M. Uren-Webster, C. Lewis, A. L. Filby, G. C. Paull, and E. M. Santos, “Mechanisms of toxicity of di(2-ethylhexyl) phthalate on the reproductive health of male zebrafish,” Aquatic Toxicology, vol. 99, no. 3, pp. 360–369, 2010.
- B. Corradetti, A. Stronati, L. Tosti, G. Manicardi, O. Carnevali, and D. Bizzaro, “Bis-(2-ethylexhyl) phthalate impairs spermatogenesis in zebrafish (Danio rerio),” Reproductive Biology, vol. 13, no. 3, pp. 195–202, 2013.
- Y. Kazeto, R. Goto-Kazeto, and J. M. Trant, “Membrane-bound progestin receptors in channel catfish and zebrafish ovary: changes in gene expression associated with the reproductive cycles and hormonal reagents,” General and Comparative Endocrinology, vol. 142, no. 1-2, pp. 204–211, 2005.
- Y. Ma, J. Han, Y. Guo et al., “Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol,” Aquatic Toxicology, vol. 106-107, pp. 173–181, 2012.
- C. Liu, L. Yu, J. Deng, P. K. S. Lam, R. S. S. Wu, and B. Zhou, “Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish,” Aquatic Toxicology, vol. 93, no. 2-3, pp. 131–137, 2009.
- M. Naderi, M. Y. L. Wong, and F. Gholami, “Developmental exposure of zebrafish (Danio rerio) to bisphenol-S impairs subsequent reproduction potential and hormonal balance in adults,” Aquatic Toxicology, vol. 148, pp. 195–203, 2014.
- S. Örn, P. L. Andersson, L. Förlin, M. Tysklind, and L. Norrgren, “The impact on reproduction of an orally administered mixture of selected PCBs in zebrafish (Danio rerio),” Archives of Environmental Contamination and Toxicology, vol. 35, no. 1, pp. 52–57, 1998.
- I. Figa-Talamanca, V. Dell'Orco, A. Pupi et al., “Fertility and semen quality of workers exposed to high temperatures in the ceramics industry,” Reproductive Toxicology, vol. 6, no. 6, pp. 517–523, 1992.
- J.-M. Mur, P. Wild, R. Rapp, J.-P. Vautrin, and J.-P. Coulon, “Demographic evaluation of the fertility of aluminium industry workers: influence of exposure to heat and static magnetic fields,” Human Reproduction, vol. 13, no. 7, pp. 2016–2019, 1998.
- Á. Vargas, E. Bustos-Obregón, and R. Hartley, “Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin,” Biological Research, vol. 44, no. 2, pp. 161–167, 2011.
- R. S. S. Wu, B. S. Zhou, D. J. Randall, N. Y. S. Woo, and P. K. S. Lam, “Aquatic hypoxia is an endocrine disruptor and impairs fish reproduction,” Environmental Science & Technology, vol. 37, no. 6, pp. 1137–1141, 2003.
- C. A. Landry, S. L. Steele, S. Manning, and A. O. Cheek, “Long term hypoxia suppresses reproductive capacity in the estuarine fish, Fundulus grandis,” Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, vol. 148, no. 2, pp. 317–323, 2007.
- P. Thomas, M. S. Rahman, I. A. Khan, and J. A. Kummer, “Widespread endocrine disruption and reproductive impairment in an estuarine fish population exposed to seasonal hypoxia,” Proceedings of the Royal Society of London B: Biological Sciences, vol. 274, no. 1626, pp. 2693–2701, 2007.
- R. M. K. Yu, D. L. H. Chu, T.-F. Tan et al., “Leptin-mediated modulation of steroidogenic gene expression in hypoxic zebrafish embryos: implications for the disruption of sex steroids,” Environmental Science & Technology, vol. 46, no. 16, pp. 9112–9119, 2012.
- D. Martinovic, D. L. Villeneuve, M. D. Kahl, L. S. Blake, J. D. Brodin, and G. T. Ankley, “Hypoxia alters gene expression in the gonads of zebrafish (Danio rerio),” Aquatic Toxicology, vol. 95, no. 4, pp. 258–272, 2009.
- K. H. Lo, M. N. Y. Hui, R. M. K. Yu, R. S. S. Wu, and S. H. Cheng, “Hypoxia impairs primordial germ cell migration in zebrafish (Danio rerio) embryos,” PLoS ONE, vol. 6, no. 9, Article ID e24540, 2011.
- L. Bujan, M. Daudin, J.-P. Charlet, P. Thonneau, and R. Mieusset, “Increase in scrotal temperature in car drivers,” Human Reproduction, vol. 15, no. 6, pp. 1355–1357, 2000.
- Y. Lue, A. P. S. Hikim, C. Wang, M. Im, A. Leung, and R. S. Swerdloff, “Testicular heat exposure enhances the suppression of spermatogenesis by testosterone in rats: the ‘two-hit’ approach to male contraceptive development,” Endocrinology, vol. 141, no. 4, pp. 1414–1424, 2000.
- E. K. Sheiner, E. Sheiner, R. D. Hammel, G. Potashnik, and R. Carel, “Effect of occupational exposures on male fertility: literature review,” Industrial Health, vol. 41, no. 2, pp. 55–62, 2003.
- K. D. Poss, A. Nechiporuk, K. F. Stringer, C. Lee, and M. T. Keating, “Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1,” Genes & Development, vol. 18, no. 13, pp. 1527–1532, 2004.
- C. Pieau, M. Dorizzi, and N. Richard-Mercier, “Temperature-dependent sex determination and gonadal differentiation in reptiles,” in Genes and Mechanisms in Vertebrate Sex Determination, pp. 117–141, Springer, Berlin, Germany, 2001.
- A. Luzio, D. Santos, A. A. Fontaínhas-Fernandes, S. M. Monteiro, and A. M. Coimbra, “Effects of 17α-ethinylestradiol at different water temperatures on zebrafish sex differentiation and gonad development,” Aquatic Toxicology, vol. 174, pp. 22–35, 2016.
- N. Villamizar, L. Ribas, F. Piferrer, L. M. Vera, and F. J. Sánchez-Vázquez, “Impact of daily thermocycles on hatching rhythms, larval performance and sex differentiation of zebrafish,” PLoS ONE, vol. 7, no. 12, Article ID e52153, 2012.
- M. Hagedorn and V. L. Carter, “Zebrafish reproduction: revisiting in vitro fertilization to increase sperm cryopreservation success,” PLoS ONE, vol. 6, no. 6, Article ID e21059, 2011.
- A. Nagabhushana and R. K. Mishra, “Finding clues to the riddle of sex determination in zebrafish,” Journal of Biosciences, vol. 41, no. 1, pp. 145–155, 2016.
- N. P. Quinn and J. D. Ackerman, “The effect of near-bed turbulence on sperm dilution and fertilization success of broadcast-spawning bivalves,” Limnology and Oceanography: Fluids and Environments, vol. 1, no. 1, pp. 176–193, 2011.
- N. P. Quinn and J. D. Ackerman, “Biological and ecological mechanisms for overcoming sperm limitation in invasive dreissenid mussels,” Aquatic Sciences, vol. 74, no. 3, pp. 415–425, 2012.
- M. Reichard, S. C. Le Comber, and C. Smith, “Sneaking from a female perspective,” Animal Behaviour, vol. 74, no. 4, pp. 679–688, 2007.
- R. L. Norman, “Effects of corticotropin-releasing hormone on luteinizing hormone, testosterone, and cortisol secretion in intact male rhesus macaques,” Biology of Reproduction, vol. 49, no. 1, pp. 148–153, 1993.
- K.-I. Maeda and H. Tsukamura, “Neuroendocrine mechanism mediating fasting-induced suppression of luteinizing hormone secretion in female rats,” Acta Neurobiologiae Experimentalis, vol. 56, no. 3, pp. 787–796, 1996.
- H. F. Erden, I. H. Zwain, H. Asakura, and S. S. C. Yen, “Corticotropin-releasing factor inhibits luteinizing hormone-stimulated P450c17 gene expression and androgen production by isolated thecal cells of human ovarian follicles,” The Journal of Clinical Endocrinology and Metabolism, vol. 83, no. 2, pp. 448–452, 1998.
- J. B. Phogat, R. F. Smith, and H. Dobson, “Effect of adrenocorticotrophic hormone () on ovine pituitary gland responsiveness to exogenous pulsatile GnRH and oestradiol-induced LH release in vivo,” Animal Reproduction Science, vol. 55, no. 3-4, pp. 193–203, 1999.
- M. Klimek, W. Pabian, B. Tomaszewska, and J. Kolodziejczyk, “Levels of plasma ACTH in men from infertile couples,” Neuroendocrinology Letters, vol. 26, no. 4, pp. 347–350, 2005.
- L. Ren, X. Li, Q. Weng et al., “Effects of acute restraint stress on sperm motility and secretion of pituitary, adrenocortical and gonadal hormones in adult male rats,” Journal of Veterinary Medical Science, vol. 72, no. 11, pp. 1501–1506, 2010.
- T. E. Orr and D. R. Mann, “Role of glucocorticoids in the stress-induced suppression of testicular steroidogenesis in adult male rats,” Hormones and Behavior, vol. 26, no. 3, pp. 350–363, 1992.
- G. P. Chrousos, D. J. Torpy, and P. W. Gold, “Interactions between the hypothalamic-pituitary-adrenal axis and the female reproductive system: clinical implications,” Annals of Internal Medicine, vol. 129, no. 3, pp. 229–240, 1998.
- E. D. Kirby, A. C. Geraghty, T. Ubuka, G. E. Bentley, and D. Kaufer, “Stress increases putative gonadotropin inhibitory hormone and decreases luteinizing hormone in male rats,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 27, pp. 11324–11329, 2009.
- R. Patiño, M. R. Wainscott, E. I. Cruz-Li et al., “Effects of ammonium perchlorate on the reproductive performance and thyroid follicle histology of zebrafish,” Environmental Toxicology and Chemistry, vol. 22, no. 5, pp. 1115–1121, 2003.
- S. Mukhi and R. Patiño, “Effects of prolonged exposure to perchlorate on thyroid and reproductive function in zebrafish,” Toxicological Sciences, vol. 96, no. 2, pp. 246–254, 2007.
- K. Ji, S. Hong, Y. Kho, and K. Choi, “Effects of bisphenol S exposure on endocrine functions and reproduction of zebrafish,” Environmental Science & Technology, vol. 47, no. 15, pp. 8793–8800, 2013.
- R. V. Kuiper, E. J. Van Den Brandhof, P. E. G. Leonards, L. T. M. Van Der Ven, P. W. Wester, and J. G. Vos, “Toxicity of tetrabromobisphenol A (TBBPA) in zebrafish (Danio rerio) in a partial life-cycle test,” Archives of Toxicology, vol. 81, no. 1, pp. 1–9, 2007.
- A. N. Haldén, J. R. Nyholm, P. L. Andersson, H. Holbech, and L. Norrgren, “Oral exposure of adult zebrafish (Danio rerio) to 2,4,6-tribromophenol affects reproduction,” Aquatic Toxicology, vol. 100, no. 1, pp. 30–37, 2010.
- H. C. Reinardy, J. R. Syrett, R. A. Jeffree, T. B. Henry, and A. N. Jha, “Cobalt-induced genotoxicity in male zebrafish (Danio rerio), with implications for reproduction and expression of DNA repair genes,” Aquatic Toxicology, vol. 126, pp. 224–230, 2013.
- F. Brion, C. R. Tyler, X. Palazzi et al., “Impacts of 17β-estradiol, including environmentally relevant concentrations, on reproduction after exposure during embryo-larval-, juvenile- and adult-life stages in zebrafish (Danio rerio),” Aquatic Toxicology, vol. 68, no. 3, pp. 193–217, 2004.
- J. P. Nash, D. E. Kime, L. T. M. Van der Ven et al., “Long-term exposure to environmental concentrations of the pharmaceutical ethynylestradiol causes reproductive failure in fish,” Environmental Health Perspectives, vol. 112, no. 17, pp. 1725–1733, 2004.
- C. Schäfers, M. Teigeler, A. Wenzel, G. Maack, M. Fenske, and H. Segner, “Concentration- and time-dependent effects of the synthetic estrogen, 17α-ethinylestradiol, on reproductive capabilities of the zebrafish, Danio rerio,” Journal of Toxicology and Environmental Health, Part A: Current Issues, vol. 70, no. 9, pp. 768–779, 2007.
- C. Liu, J. Deng, L. Yu, M. Ramesh, and B. Zhou, “Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol,” Aquatic Toxicology, vol. 96, no. 1, pp. 70–76, 2010.
- M. Galus, J. Jeyaranjaan, E. Smith, H. Li, C. Metcalfe, and J. Y. Wilson, “Chronic effects of exposure to a pharmaceutical mixture and municipal wastewater in zebrafish,” Aquatic Toxicology, vol. 132-133, pp. 212–222, 2013.
- M. Galus, N. Kirischian, S. Higgins et al., “Chronic, low concentration exposure to pharmaceuticals impacts multiple organ systems in zebrafish,” Aquatic Toxicology, vol. 132-133, pp. 200–211, 2013.
- K. Ji, X. Liu, S. Lee et al., “Effects of non-steroidal anti-inflammatory drugs on hormones and genes of the hypothalamic-pituitary-gonad axis, and reproduction of zebrafish,” Journal of Hazardous Materials, vol. 254-255, no. 1, pp. 242–251, 2013.
- M. Galus, S. Rangarajan, A. Lai, L. Shaya, S. Balshine, and J. Y. Wilson, “Effects of chronic, parental pharmaceutical exposure on zebrafish (Danio rerio) offspring,” Aquatic Toxicology, vol. 151, pp. 124–134, 2014.
- R. Nourizadeh-Lillabadi, J. L. Lyche, C. Almaas et al., “Transcriptional regulation in liver and testis associated with developmental and reproductive effects in male zebrafish exposed to natural mixtures of persistent organic pollutants (POP),” Journal of Toxicology and Environmental Health, Part A: Current Issues, vol. 72, no. 3-4, pp. 112–130, 2009.
- T. Daouk, T. Larcher, F. Roupsard et al., “Long-term food-exposure of zebrafish to PCB mixtures mimicking some environmental situations induces ovary pathology and impairs reproduction ability,” Aquatic Toxicology, vol. 105, no. 3-4, pp. 270–278, 2011.
- R. H. M. M. Schreurs, J. Legler, E. Artola-Garicano et al., “In vitro and in vivo antiestrogenic effects of polycyclic musks in zebrafish,” Environmental Science & Technology, vol. 38, no. 4, pp. 997–1002, 2004.
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