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BioMed Research International
Volume 2016 (2016), Article ID 9821767, 9 pages
http://dx.doi.org/10.1155/2016/9821767
Research Article

Anticerebral Ischemia-Reperfusion Injury Activity of Synthesized Puerarin Derivatives

1Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China
2Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China
3Harbin University of Commerce, Harbin 150076, China

Received 3 June 2016; Revised 2 August 2016; Accepted 29 August 2016

Academic Editor: Luca Cucullo

Copyright © 2016 Yubin Ji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

When cerebral ischemia-reperfusion injury happened in patients, multiple pathological processes occur, such as leukocyte infiltration, platelet, and complement activation, which would result in cognitive dysfunction and inflammation. Puerarin has shown protective effect on injury of neural cell. In order to enhance this protective effect of puerarin, puerarin derivatives with different values were designed and synthesized. The original phenolic hydroxyl in the puerarin molecules was substituted in order to change the blood-brain barrier permeability and thus enhance the efficacy for preventing cerebral ischemia/reperfusion injury. And the structure of the newly synthesized molecules was confirmed by 1H NMR spectroscopy and mass spectrometry. The mouse model of cerebral artery ischemia/reperfusion injury was established to test the anticerebral ischemia-reperfusion injury activity of the puerarin derivatives. The assays of the water maze, Y maze, brain cortex Ca2+-Mg2+-ATP enzyme, and iNOS enzyme activity were performed in this mouse model. The results showed that puerarin derivative P1-EA and P2-EA were resulting in an increased lipophilicity that enabled the derivatives to pass more efficiently through the blood-brain barrier, thus, improving the protective effects against cerebral ischemia/reperfusion injury. Therefore, derivatives of puerarin may serve as promising approach to improve neuron function in ischemia-reperfusion brain injury-related disorders.