Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production
Optimization of UGT72B14-opt expression. (a) Protein expression at 10 h after induction with IPTG at varying OD600. (b) Growth profile of recombinant strain during induction. (c) Time courses of protein expression with IPTG induction at OD600 = . (d) Time courses of protein expression with IPTG induction at OD600 = . Lane M: protein molecular weight markers. Lanes I–IV: UGT72B14-opt expression at OD600 ~ 0.1, 0.3, 0.6, and 0.9 with 10 h after induction. Lanes 1–7: UGT72B14-opt expression at 0, 2, 4, 6, 8, 9, and 10 h after induction. Cell pellets were resuspended, the OD600 was adjusted to , and 15 μL was used for SDS-PAGE analysis. Target protein is indicated by arrows. Data are presented as mean of three biological replicates, and error bar represents the SD.
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