Evaluation of Two Supplemented Culture Media for Long-Term, Room-Temperature Preservation of Streptococcus pneumoniae Strains

Table 1

Phenotypic and genetic characteristics of S. pneumoniae strains.

Strains	Code	Serotype	MLST type	Related international clone	Source	Resistance pattern	Transposons
First group	H2	11	ND	ND	CSF	P, T/S	ND
	61	NT	ND	ND	Conjunctivitis	T/S	ND
	EF 28	23 F	ND	ND	Nasopharynx	P	ND
	EF 33	NT	ND	ND	Nasopharynx	E, T, T/S	ND
Second group	I-121	6B	90	-2	Nasopharynx	P, CRO, CXM, MEM, C, CD, E, T, T/S	Tn5253 plus Tn917
	I-15	6B	90	-2	Nasopharynx	P, CXM, MEM, CD, E, T, T/S	Tn5253 plus Tn917
	H-183	6B	135	No	Nasopharynx	P, CD, E, T, T/S	Tn3872
	H-222	6B	135	No	Nasopharynx	P, C, T/S	None
	I-206	14	15	SLV -9	Nasopharynx	CD, E, T/S	Tn3872
	H-12	14	2563	DLV -9	Nasopharynx	CD, E, T	Tn6002
	I-196	23F	5165	SLV -1	Nasopharynx	P, CXM, MEM, C, CD, E, T, T/S	Tn3872
	I-246	23F	81	-1	Nasopharynx	P, CRO, CTX, CXM, FEP, MEM, C, E, T, T/S	Tn2009
	I-176	23F	242	-15	Nasopharynx	CD, E, T, T/S	Tn3872
	H-59	23F	242	-15	Nasopharynx	E, T, T/S	Tn2009
	I-120	23 A	42	DLV -4	Nasopharynx	CD, E, T, T/S	Tn6002
	I-248	19F	5167	SLV -14	Nasopharynx	P, CXM, ETP, MEM, CD, E, T, T/S	Tn2010
	H-123	19 A	320	DLV -14	Nasopharynx	P, CTX, CXM, ETP, MEM, CD, E, T, T/S	Tn2010
	I-29	NT	5168	SLV1106 NCC1	Nasopharynx	P, E, T, T/S	Tn2009
	I-34	NT	4837	DLV1106 NCC1	Nasopharynx	P, E, T, T/S	Tn2009

: multilocus sequence typing, : null capsule clade, and PMEN: pneumococcal molecular epidemiology network clone; isolated from cerebrospinal fluid of a child with meningitis with PNC MIC 0.25 µg/mL, isolated from a child with purulent conjunctivitis, isolated from nasopharyngeal carriers aged 0–5 years, MIC level of 0.25 µg/mL, MIC level of 2 µg/mL, and MIC level of 0.50 µg/mL. NT: nontypeable strains, ND: not done, SLV: single-locus variant, DLV: double-locus variant, P: penicillin, CRO: ceftriaxone, CTX: cefotaxime, CXM: cefuroxime, FEP: cefepime, ETP: ertapenem, MEM: meropenem, C: chloramphenicol, CD: clindamycin, E: erythromycin, T: tetracycline, and T/S: trimethoprim-sulfamethoxazole.