Research Article
Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naïve Schwann Cells in the Sciatic Nerve Injury Model
Figure 3
Immunofluorescence stainings for P0, laminin, and Caspr at 12-week transplantation. (a) Dual fluorescence images for GFP (green) and P0 immunofluorescence staining (red). In the eGFP-SCs group, cytosolic expression of GFP proteins wrapped P0-expressing compact myelin sheath outwardly and segment-like structures of GFP fluorescence were coincided with P0-lacking sites, suggesting nodes of Ranvier structures. Several P0-negative holes were detected, suggesting a vascular structure (arrows) and endoneurium-like structure (arrowheads). In the eGFP-SCs group, GFP fluorescence also wrapped the P0-expressing hollow tube-like structures outwardly and tightly. (b) Dual fluorescence images for GFP (green) and laminin immunofluorescence staining (red). In the eGFP-SCs group, laminin immunoreactivities were colocalized with the outer boundary of GFP-expressing cells; segment-like structures of GFP fluorescence were also coincided with laminin immunoreactivities. Colocalization of GFP fluorescence and laminin immunoreactivity was also found in the eGFP-SCs group. (c) Dual fluorescence images for GFP (green) and Caspr immunofluorescence staining (red, arrows). In the eGFP-SCs group, doublets of Caspr for myelin sheath at the node of Ranvier were enclosed by two GFP-expressing cells (arrowheads). This symmetric enclosure was also found in the eGFP-SCs group.
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