Biochemical and Molecular Analysis of the Hb Lepore Boston Washington in a Syrian Homozygous Child

Figure 3

Nucleotide sequencing of the hybrid δβ-globin gene showing the crossover breakpoint that occurred between exon 2 and IVS 2. The 86 and 87 codons (left box) belong to the δ-gene, whereas the downstream region, starting from IVS 2 nt 8 (right box), belongs to the β-globin gene. The 58 bp region spanning from codon 88 to IVS 2 nt 7, in which the two genes share the same sequence, cannot be unequivocally attributed to any of them. The strings of capital letters above the base calling show the reference sequences of the δ- and β-globin genes (GenBank accession number NG_000007.3).