Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis

Figure 3

Histological finding and assessment of ECM deposition. (a) Representative images of H&E (upper) and Masson’s Trichrome (lower) stained sections of lung tissue of control (Cont), PTX-treated (PTX), irradiated (IR), and irradiated and PTX-treated rats 16 weeks after irradiation. Scale bars represent 200 µm. (b) Quantification of lung fibrosis in the four treatment groups in (a) expressed as modified Ashcroft score. Data are expressed as the mean ± SEM . versus control group; versus IR group. (c) Western analysis and (d) quantification of FN and PAI-1 expression in lungs of rats in the four indicated treatment groups. Band densities were normalized to those of β-actin. Data were normalized to control and expressed as the mean ± SEM . versus control group; versus IR group. H&E, hematoxylin and eosin staining; PTX, pentoxifylline; FN, fibronectin; PAI-1, plasminogen activator inhibitor-1.

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