Proliferative and Invasive Effects of Progesterone-Induced Blocking Factor in Human Glioblastoma Cells
P4 regulates PIBF expression in glioblastoma cells. PIBF gene expression was evaluated by RT-PCR in U87 cells treated with vehicle (V, cyclodextrin 0.02%) and P4 (10 nM) for 6, 12, and 24 h. (a) Representative image of PIBF gene expression (530 bp) at different times of treatment and the respective expression control gene 18S rRNA (150 bp). (b) Densitometric analysis of three independent experiments; PIBF expression values were normalized to those of the control gene 18S rRNA. The data are expressed as the mean ± S.E.M. with ; versus vehicle.