Calcium Ionophore, Calcimycin, Kills Leishmania Promastigotes by Activating Parasite Nitric Oxide Synthase

Figure 1

Viability of L. major promastigotes treated with calcimycin. (a) The percentage of viable L. major promastigotes in cultures treated with calcimycin and amphotericin B in comparison with untreated culture (taken as 100%). Data are presented as mean ± SD (three independent experiments; each assay was performed in triplicate). (b) Numbers of L. major promastigotes were counted using Z2 COULTER COUNTER. The column “0 μM, before treatment” reflects starting promastigote concentration in the beginning of coincubation with calcimycin. A column labeled 0 μM shows number of nontreated promastigotes after 48 hours of incubation. Columns labeled 0.5 μM, 1.0 μM, and 2.0 μM show promastigote numbers after another 48 hours of coincubation with corresponding concentrations of calcimycin. Data are presented as mean ± SD (six independent experiments; each assay was performed in triplicate). Promastigote numbers were compared using Mann–Whitney test. corresponds to ; corresponds to .

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