Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca²⁺ Signaling In Vitro

Figure 5

CaMKII, PKC, and PLC expression following the intervention with various ESW energy levels. ESW increases the mRNA levels of CaMKII and PLC and decreases the mRNA levels of PKC at different time point in BMMSCs. (a) Western blot assay of CaMKII, PKC, and PLC receptor protein expression. GAPDH served as loading control. BMMSCs were exposed to ESW (0.2 bar, 0.4 bar, and 0.6 bar, 6 Hz, and 1000 shots) and detected at various time points as indicated. (b, c, d) BMMSCs were exposed to ESW (0.2 bar, 0.4 bar, and 0.6 bar, 6 Hz, and 1000 shots) and detected at various time points as indicated. The mRNA was extracted from the cell lysates, and Q-RT-PCR with the primers of CaMKII, PKC, PLC, and GAPDH (internal control) was performed as described in Methods. The quantitative data were shown as ().

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