High Glucose Promotes CD36 Expression by Upregulating Peroxisome Proliferator-Activated Receptor γ Levels to Exacerbate Lipid Deposition in Renal Tubular Cells

Figure 2

Effects of LY294002 on HG-induced CD36 expression. HK-2 cells were cultured with normal glucose (5.6 mM, NG), NG plus LY294002 (15 uM), high glucose (30 mM, HG), or HG plus LY294002 for 48 h. CD36 protein levels were determined by western blotting. Band intensities were normalized to β-actin band intensity using densitometry. The histogram represented the normalized intensities of proteins from three experiments. The data were represented as the means ± SD (a). CD36 mRNA levels were measured by RT-qPCR. β-Actin served as a reference gene. The results from three independent experiments were represented as means ± SD (b). versus NG; versus HG. Immunofluorescence staining of CD36 was shown in (c).

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