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BioMed Research International
Volume 2017, Article ID 1432970, 8 pages
https://doi.org/10.1155/2017/1432970
Research Article

Influence of Supplementation of Vegetable Oil Blends on Omega-3 Fatty Acid Production in Mortierella alpina CFR-GV15

1Microbiology & Fermentation Technology Department, CSIR-Central Food Technological Research Institute, Mysore, India
2Resource Centre, CSIR-Central Food Technological Research Institute, Habsiguda, Hyderabad, India

Correspondence should be addressed to Govindarajulu Venkateswaran; moc.liamg@irtfctaknev

Received 23 November 2016; Revised 14 February 2017; Accepted 12 March 2017; Published 29 March 2017

Academic Editor: Fabiano J. Contesini

Copyright © 2017 Ganesan Vadivelan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives of this study were designed for improved production of mycelial omega-3 fatty acids with particular reference to EPA and DHA from the oleaginous fungus Mortierella alpina CFR-GV15 under submerged low temperatures fermentation supplemented with linseed oil and garden cress oil as an additional energy source. The fungus was grown at 20°C temperature for four days initially followed by 12°C temperature for next five days. The basal medium contained starch, yeast extract, and a blend of linseed oil (LSO) and garden cress oil (GCO) in the ratio 1 : 1. Results of the study revealed that, after nine days of total incubation period, the enhancement of biomass was up to 16.7 g/L dry weight with a total lipid content of 55.4% (v/w). Enrichment of omega-3 fatty acids indicated a significant increase in fatty acid bioconversion (ALA %, EPA %, and DHA %) by 2.5-fold. The two-stage temperature cultivation alters the fatty acid profile due to activation of the desaturase enzyme in the cellular levels due to which arachidonic acid (AA) content reduced significantly. It can be concluded that Mortierella alpina CFR-GV15 is a fungal culture suitable for commercial production of PUFAs with enriched EPA and DHA.