Influence of Supplementation of Vegetable Oil Blends on Omega-3 Fatty Acid Production in Mortierella alpina CFR-GV15

Table 3

Effect of individual vegetable and blended oil supplementation as carbon source on M. alpina CFR-GV15 on biomass, total lipid, and omega-3 fatty acid production.

Oil supplement	Dry biomass; (g/L)	Total lipid; (% w/w)	Fatty acid content (% w/w)
			18:3 (n-3)	20:4 (n-6)	20:5 (n-3)	22:6 (n-3)
GY medium
MO			ND
RBO			ND
SBO			ND
SFO			ND
GCO
CO
LSO
Blend oil supplement (2% level)
LSO + SFO
LSO + SBO
LSO + RBO
LSO + MO
LSO + GCO
LSO + CO
GCO + CO

Culture conditions: GY medium: glucose and yeast extract 2 : 1 ratio; pH 6.5; 230 rpm; cultivation period 7-day values of means SD (control). Optimized medium carbon source: 1% carbon and 0.5% yeast extract; 1% KNO₃; 0.1% KH₂PO₄ and MgSO₄·7H₂O 0.05%; additional 2% individual vegetable and blended oil supplementation; pH 6.5; 230 rpm; cultivation period: 9-day values of means ± SD, n-3. Values in the same column that do not share the same alphabetic superscripts are significantly different at levels according to Duncan’s multiple range tests.