Prokaryotic Expression of α-13 Giardin Gene and Its Intracellular Localization in Giardia lamblia

Figure 4

Confirmation of specificity of anti-α13 giardin antibodies. (a) Western blotting of recombinant α-13 giardin proteins. 1, reactivity of the anti-α13-giardin antibodies with the recombinant proteins and 2, control with preimmune serum. (b) Western blotting of G. lamblia trophozoite lysates separated by SDS-PAGE (12% acrylamide). M, standard protein marker; 1, Coomassie stain of total trophozoite lysates; 2, specific reactivity of the anti-α13 giardin antibodies with trophozoite lysates; and 3, control with preimmune serum.

(a)

(b)