Dexmedetomidine Protects Cardiomyocytes against Hypoxia/Reoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling

Figure 3

TLR4 knock-down by TLR4 siRNA transfection protects cardiomyocytes against H/R injury. TLR4 protein expression was detected in cells transfected with TLR4 siRNA for 24 h by Western blot analysis (a). GAPDH was used as a loading control. Data are expressed as means ± SEM ( = 3 per group). < 0.01 versus Lip2000 group. Viability of H9C2 cells and rat neonatal cardiomyocytes was measured by MTT (b, c) and LDH activity (d, e). Data are expressed as means ± SEM ( = 5 per group). < 0.05, < 0.01 versus control group; < 0.05, < 0.01 versus H/R group. D, DEX.

(a) H9C2 cells transfected with TLR4 siRNA

(b) H9C2 cells

(c) Neonatal cardiomyocytes

(d) H9C2 cells

(e) Neonatal cardiomyocytes