Modeling Beta-Traces for Beta-Barrels from Cryo-EM Density Maps

Figure 1

Secondary structure detection from density maps. Left: A density map (gray) was simulated at 10 Å resolution using the atomic structure of protein 3GP6 (PDB ID) in (a), 1TIM (PDB ID) in (b), and EMAN software [38]. Middle: helices (colored lines) and β-sheets (blue voxels) are detected from the density maps using SSETracer [24]. Right: The detected α-helixes and β-sheets are superimposed with the atomic structure in (a) and (b), respectively. Each atomic structure contains a β-barrel. Figure 1(a) is reproduced with permission from IEEE [39].

(a)

(b)