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BioMed Research International
Volume 2017 (2017), Article ID 1832853, 10 pages
Research Article

Anti-Inflammatory Effect of Dialyzable Leukocyte Extract in Autoimmune Prostatitis: Evaluation in Animal Model

1ENMH, Instituto Politécnico Nacional, Ciudad de México, Mexico
2CBG, Instituto Politécnico Nacional, Cd. Reynosa, TAMPS, Mexico
3Hospital General Milpa Alta, Ciudad de México, Mexico
4Hospital de Traumatología, IMSS, Ciudad de México, Mexico
5Farmainmune, Ciudad de México, Mexico

Correspondence should be addressed to D. Guillermo PĂ©rez Ishiwara

Received 3 November 2016; Revised 13 January 2017; Accepted 14 February 2017; Published 12 March 2017

Academic Editor: Humberto H. Lara

Copyright © 2017 Carlos Pérez-Alvarado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To evaluate the anti-inflammatory properties of Dialyzable Leukocyte Extract (DLE) in a murine model of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods. Histopathological characterization, prostatein Enzyme-Linked Immunosorbent Assay, and immunohistochemical analysis for CD45, TNF-α, IFN-γ, IL-6, IL-17, and IL-4 molecules were done in prostatic Wistar rats treated with DLE, placebo, or Dexamethasone. Results. Histopathological analysis of animals induced to prostatitis showed inflammatory infiltrate, mainly constituted by leucocytes and mast cells as well as Benign Prostatic Hyperplasia. Serum prostatein concentrations were 14 times higher than those displayed by healthy animals. After DLE and Dexamethasone treatments, the inflammatory infiltrate decreased; the tissue morphology was similar to that of a normal prostate, and the prostatein decreased to the basal levels of healthy animals. DLE treatment produced a decreased expression of the cell surface marker CD45 and the proinflammatory cytokines TNF-α, IFN-γ, IL-6, and IL-17. On the other hand, the expression of anti-inflammatory cytokine IL-4 increased in both the Dexamethasone and DLE groups. Conclusion. DLE is able to modulate the inflammatory response in Experimental Autoimmune Prostatitis (EAP).