Protective Effect of a Polyherbal Aqueous Extract Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Roots), and Smilax glabra (Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes

Table 2

The effect of the decoction at a dose range of 100–600 µg/mL on micronuclei induction in vitro by bleomycin (40 µg/mL) in cultured human lymphocytes. The values are mean ± SD per 1000 binucleated cells per individual of a sample population of 6 individuals. Nuclear division index (NDI) was expressed as mean ± SD for each experimental groups.

Experimental groups	Concentrations; (μg/mL)	MN/1000 BL; (mean ± SD)	MN% inhibition	NDI; (mean ± SD)
Negative control		0.00967 ± 0.00252		1.64333 ± 0.10786
Decoction control	Dec. 100; 300; 600	0.01167 ± 0.00252; 0.00983 ± 0.00241; 0.01187 ± 0.00213		1.66333 ± 0.10693
Bleomycin control	BLM 40	0.08833 ± 0.00603
Test (decoction + bleomycin)	BLM 40; Dec. 100; 300; 600		71.69; 76.23; 72.83	1.6 ± 0.12166